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this type on all media, invariably produced a whitish, spreading growth upon 
potato in 24 hours. 
Tataroff’s organism having been isolated from fresh water and this closely 
allied form from a salt water clam, it seems possible that the variations noted— 
namely, slower growth in litmus milk and the absence of growth on potato were 
due to lack of salt in these media. 
It is hoped that this will be confirmed at a later date. 
5. Pseudomonas myae. 
Though this form had well-defined characteristics it has not been discovered 
in the available classifications and descriptions. In many particulars it is 
suggestive of the Pseudomonas oogenes of Migula which has been found ‘n eggs. 
In form it was a short rod with one polar flagellum. It was gram-negative. 
On gelatin plates white colonies developed in between.2 and 3 days. Lique- 
faction was bowl-shaped, a dense white deposit occupying the central point in 
the depression. Around this, a less dense, uniform turbidity gave the remainder 
of the bowl a frosty-white appearance. Observed microscopically the small 
deep-set colonies were found to be very dense, spherica’, granular masses. The 
larger colonies seemed often to be built concentrically about such a colony as a 
central nucleus. Other large colonies were merely grumose in the centre, a 
loosely granulated zone occupying a comparatively wide area between the 
central portion and the margin. 
On agar plates, small, deep-set, pyramidal colonies were numerous. Surface 
colonies were of the round, moist, cream-coloured type. Microscopically, they 
were granular without projections and dense in the centre. Occasionally 
branching and budding of the colonies was observed. 
In litmus milk the litmus was quickly reduced. Clotting and digestion 
followed rapidly. _They whey was perfectly clear without a pellicle, while the 
clot became deep pink in colour. 
Ps. myae was the only one of the blackening organisms which fermented 
lactose. 
In gelatin stab a crateriform-filiform liquefaction was well-developed at the 
end of 24 hours. The liquefied area gradually widened, reaching the sides of the 
tube in 3 or 4 days. A statiform area thus appeared above an ever-widening 
central turbid column. In ten days the gelatin was completely liquefied, with 
a heavy, creamy, viscid sediment. 
In nutrient broth there was a light pellicle, marked turbidity and a viscid 
sediment. Ly 
The organism grew delicately upon potato, the growth appearing as a 
narrow white film along the line of inoculation. It was at first mucoid but soon 
became flattened, dry and shining, remaining unchanged at this stage for weeks. 
The indol test gave a negative result. 
6. Pseudomonas sp? 
Another form, having all of the features mentioned in the general description, 
was observed. Though it was very motile, the number of flagella was not 
determined. 
This was a small rod which had a tendency to bipolar staining. It frequently 
formed short chains. It was gram-negative. 
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