AFFECTED SALMON, MIRAMIOHI HATCH ERT 151 



SESSIONAL PAPER No. 38a 



fish a large number of colonies were isolated. These colonies were lettered and 

 numbered, and besides those here described a large number of other colonies were 

 isolated, which were compared and found similar to those mentioned by letter and 

 number. 



Fish No. 1. Appearance normal, with the exception of a few patches of diseased 

 skin around the head. On opening, the organs appeared normal. Plates were made 

 from milt, liver, swimming bladder, kidney, heart's blood. In all cases the material 

 was transferred to sterile petri dishes and beef peptone salt water agar poured over. 

 After the plates had set they were kept at 20°C, Kesults: — 



Milt. — About 60 colonies. 

 Liver. — About 100 colonies. 



Swimming bladder. — Contained a quantity of liquid. Very large number 

 of colonies, too numerous to count. 



Heart's blood. — About 300 colonies to the oese. All these colonies wer^. 

 very similar. 



Kidneys. — About 90 colonies. 

 Four species were isolated from this fish, marked Al, A2, A3, A4. 

 Flesh near diseased skin normal in appearance. 



Fish No. 2. — ^External appearance normal except some bruises with traces of the 

 fungus development near tail and head. On opening, the liver was rather pale in 

 colour, somewhat friable, intestines empty, caeca empty. Right ovary eggs pink in 

 colour; left ovary eggs much darker in colour, almost liver-coloured. Flesh normal 

 and good colour. Same technique. One oese from each of the parts mentioned. 



Ovary. — Pink eggs. From one crushed egg 300 or 400 colonies developed. 

 A larger number from the one crushed egg from the dark red left ovary. 

 Liver. — 20 colonies. 



Heart's blood. — 60 colonies per oese, all practically identical. 

 Isolations Bl, B2, B3, B4. 



Fish No. 3. — Exterior appearance normal with the exception of a few small areas 

 discoloured visible in the skin. Flesh normal in appearance. Interior organs appar- 

 ently normal. Smears from the various organs showed bacteria. 



Heart's blood. — x\bout 250 colonies to the oese, all similar. 

 Eggs. — Innumerable colonies. Two species. 

 Liver. — 20 — 30 colonies per oese. 

 Kidneys. — 80 — 100 colonies. 

 Two isolations — Cl, 02. 



Fish No. 4. — A large fish ; much gelatinous slime around the tail. Some areas of 

 skin affected with the fungus. Flesh beneath appeared healthy. Intestines slightly 

 congested, empty. Liver dark in colour. Eggs salmon pink in colour, apparently 

 normal. SVimming bladder empty. Smears from the heart's blood liver and kidney 

 showed a number of organisms : — 



Heart's blood. — 30 — 40 colonies, all similar. 

 Liver. — 10 — 12, all similar. 

 Kidneys. — 20 colonies, all similar. 



Eggs. — About 150 per egg. This is an estimate, as a large growth had 

 occurred in the vicinity of the crushed part of the egg. 

 One isolation, Dl. 



