154 DEPARTMENT OF THE NAVAL SERVICE 



8 GEORGE V, A. 1918 



Sloped Beef Peptone Agar, Blood Heat (37-5): — 

 Little change after three days' growth. 

 The organism grew fairly well at blood heat. 

 2Jf hours, spread over about half the sloped surface. 



JfS hours, growth denser, spreading, flat, glistening, smooth, semi-opaque, 

 whiteish. No further change. 



Glucose Agar Slope: — 



24- hours, at room temperature, smooth, vigorous, whitish, moist and spread- 

 ing. Cloudiness near the growth. 



5 days^ colony more cloudy, considerable gas production and the column of 

 agar is burst apart in the middle. 



Glucose Agar Stick: — 2Jf hours. Growth vigorous over surface and pronounced 

 cloudiness from the surface to a depth of 10 mm. 



Jf8 hours. Increase in growth and a few gas bubbles appear on the line of 

 puncture. 



No further change occurs. 



Beef Broth V— 



SJ/. hourSj strong, cloudy. 



S days^ much heavier. Sediment flocculent. 



7 days^ yellowish-green appearance in the upper layer otherwise no change. 



iJunhams's Solution : — 



The organism grew well in Dunham's solution, and at the end of 5 days at 

 room temperature was tested with Ehrlich test, allowed to stand 20 minutes and 

 the results then recorded. This organism was negative to this test. No Indol. 



MilJc:— 



2Jj. hourSj no change. 



3 days, coagulated with extrusion of slight amount of whey. 



5 days, curd has become firmer, and a cheesy smell developed. 



7 days, slightly more whey extruded; 



No other change, although observed for some twenty days. 



Litmus Milk: — 



2Jf hours, no change. 



Jf8 hours, no change in constituency, but colour is changed to avellaneous.^ 

 5 days. Colour uniform, slight digestion with separated whey, soft curd, 

 yellowish ring around glass, smell disagreeable. 



3 iveeks. — Curd still undigested, whey yellowish, yellow ring, curd avellaneus, 

 few gas bubbles on shaking. 



Potato : — 



2Jf hours. Moderate, dry, slightly raised, cream-coloured growth. 

 Jf8 hours Increase of growth, dry, raised, slightly rugose, cream-yellow colour. 

 6 days. Abundantly raised, massive, rugose growth, cream colour at margins and 

 pinkish on top. Odour unpleasant and slightly pungent, resembling that on 

 milk. 

 3 weeks. No change. 



A. 2. 

 Small bacillus with rounded ends, short, often in pairs, actively motile, stains well 

 with methylene blue, and is gram negative. 



1 Chromotaxia seu Nomenclator Colorium. P. A. Saccardo. 



