156 DEPARTMEXT OF THE NAVAL SERVICE 



8 GEORGE V, A. 1918 



Litmus Milk: — 



S4- hours. l\o cliauge. 



48 hours. K"o change iu colour or consistency; on shaking numerous small gas 



bubbles appear and form a foam on surface. 

 6 days. Coagulated, moderately firm curd, liliacinus in colour. About a quarter 



of the tube is whey, and much darker in colour (atro-violaceus). 



5 weeJvS. There is a reddish ring at the surface, considerable digestion, whej' 



occupying three-quarters of the tube, isabellinus in colour. Curd flocculent, 

 avellaneus; odour slightly cheesy. 



Fotato: — 



21^ hours. Growth moderate, filiform, slightly raised, cream-yellow colour. This 

 increases, and in 



6 days growth is moderate, raised, rugose, moist, shiny; dirty cream-yellow, 



darker in centre where growth is most massive. 

 3 iveehs. No further change. 



A. 3. 



Medium-size bacillus with rounded ends, resembles A. 1 in appearance. Active 

 motile, stains well with methylene blue, and is gram negative. 



Gelatine Plates: — 



2Ji. hours. Just visible to the naked eye. Growth rapid. 



Jf8 hours. Colonies are 2-3 mm. in diameter, round. Liquefaction saucer-shaped, 

 inner ring dense, caused by deposited bacilli. With « objective the edges of 

 the colonies are fimbriate centre grumose and flocculent. Masses of the 

 bacteria can be seen in movement. 



3 days. Colonies increase to 12 mm. in diameter, saucer-shaped liquefaction, 



whitish in centre, more transparent at the margin. To the naked eye the 

 edges are entire, but with a microscope slightly fimbriate. There is a cheesy 

 smell on opening the plates. 



4 days. Plates are liquefied. 



Gelatine Stich: — 



2Jf hours. Eesembles A. 1, but slightly less growth. 



JfS hours. Line of bacteria is filiform, smooth on surface. Liquefaction strati- 

 form, 4 mm. deep. Liquefaction continues. 



10 days. Liquefaction is 1 cm. deep with medium beneath darker in colour, but 

 clear. 



J gar Plates: — 



Jf8 hours. Colonies are 1-3 mm. in diameter, round, raised, yellowish-white. With 

 S objective edges are entire, dark in centre, granular, gradually becoming 

 lighter to margin, which is clear. 



5 days. Colonies are round, white, edges entire, brownish in centre. Convex. 

 4 days. No change. 



Agar Slope, 37° C— 



2Jt howrs. Very slight growth, filiform. n- 



7 days. No further change. 



Glucose Agar Slope^ 20° : — 



A spreading, flat, white, shiny growth; agar beneath very cloudy. Cream yellow. 

 No gas. 



