158 DEPARTMENT OF TEE NAYAL SERVICE 



8 GEORGE V, A. 1918 



Agar Plates : — 



J^ hours. Colonies are filiform, glistening, raised. With § objective the colonies 



are round, dense in centre, and granular; clearer at margin, edges entire. 

 S days. Colonies slightly larger, opalescent, white. 

 No further change. 



Gelatine Agar Slope at 31° C .• — 



Little, if any, growth observed. Continuous observation for 7 days. 



Glucose Agar Slope : — 



Growth moderate, moist, shiny, slightly raised, whitish. 

 3 weeks. Agar is brown beneath the slope. 



Glucose Agar Stick: — 



Growth filiform, thin surface, growth spreading. Gas bubbles along line of punc- 

 ture. 



No further change except the agar becomes brown beneath the surface to a depth 

 of 1-2 cm. 



Beef Broth:— 



2Jf. hours. Slight clouding and sediment. 



3 days. Clouding and sediment increase slightly. 



No further change. 



Dunham's Solution: 



Grown for five days at room temperature, tested with Ehrlich test, allowed to stand 

 20 minutes and then recorded. Indol positive. 



Milk:— 



5 days. No change visible. 



6 7 days. On shaking tube a gassy foam rises to the surface. 

 10 days. Milk had coagulated, hard curd, whitish whey. 



Litmus Milk: — 



No change in appearance in 2]). hours. 



J/S hours. Abundant gas which rises to the surface in small bubbles. This was 

 noticed each day up to the sixth day, and the foam was very heavy. The milk 

 gradually coagulates and forms a blue ring down one side of the tube, remainder 

 is a firm curd adhering to the tube. Bleached cream colour. 



Potato : — 



^.4 hours. Moderate growth, filiform, slightly moist, cream coloured. 

 JfS hours. Becomes slightly rugose. 



6 days. Growth slight, slightly raised, and a dirty yellow (melleus). 

 3 weeks. No further change. 



B. 1. 



This organism on examination was found to resemble in all respects A. 1. 



B. 2. 



A small size bacillus about li times as long as wide, rounded end, frequently in 

 pairs. Actively motile, stains well with methylene blue, negative with gram. 



