AFFECTED SALMON, MIRAMICHI HATCHERY 159 



SESSIONAL PAPER No. 38a 



Gelatine Plates: — 



2^ hours. Visible to the naked eye. 



JfS hours. Punctiform, colony raised, glistening, whitish, g objective shows round, 



dense, granular colony, entire edges. 

 No further change. 



Gelatine Stick: — 



21^ hours. Growth uniform, round, filiform; no liquefaction, 



Jf8 hours. Growth uniform, no liquefaction to surface. 



S days. Slight depression at the boint of bacteria. 'No liquefaction. 



Agar plates: — 



45 hours. Uniform, 1 m.m. in diameter, round, glistening, colony. With i object- 

 ive round, dense, shading to lighter; granular, edges entire. 

 S days. Colonies are glistening and bluish white. 

 No further change. 



Agar slope 37° C. : — 



7 days. Very slight growth, one or two small colonies appearing on the surface 

 but otherwise no change. 



Glucose Agar Slope: — 



Moderate growth, spreading, flat, moist and whitish. 



Jf8 hours. A few gas bubbles appear and slight increase in growth. 



S weeks. Agar is brown underneath the slope. 



Glucose Agar Stick: — 



Filiform, slight growth on surface, gas bubbles along line of puncture. 



No further change except for browning of the agar underneath the surface. 



Beef hroth: — 



BJi. hours. Moderate growth, moderate sediment, 

 S days. Growth slightly heavier. 



5 days. Clearing. , 

 No further change. 



Dunham's Solution: — 



Grown for five days at room temperature, tested with Ehrlich test, allowed to 

 stand 20 minutes and then recorded. Indol positive. 



Litmus Milk: — 



2Jf hours. No change. 



Jf8 hours. A fine foam on the surface when tube is shaken. Colour liliaceous, no 

 coagulation. 



6 days. Much gas in foam form. , No coagulation. Colour liliaceous. Colour 



gradually bleaches. Blue ring forms on surface. Bluish whey but little 

 digestion. 



Potato : — 



2Jlf. hours. Filliform, dry, raised, colour niveus. 



6 days. Growth becomes slightly raised and more massive. 



S iveeks. No change, 



B. 3 

 Resembles in all respects A. Jf. 



