160 DEPARTMENT OF THE NAVAL SERVICE 



8 GEORGE V, A. 1918 

 B. 4. 



Ensembles in all respects A. ^. 



C. 1. 



Small to medium bacillus about twice as long as broad, slightly rounded ends. 

 Actively motile, stains somewhat unevenly with methylene blue, gram negative. 



Gelatine Plates: — 



2^ honrs. Colonies visible to the naked eye. 



J^8 hours. Colonies punctiform, round, white, raised and glistening. § objective 



round, evenly dense and granular with entire edges. 

 No further change. 



Gelatine SticJc: — 



Growth uniform, line of puncture filiform, 4 gas bubbles along line of puncture. 

 10 days. Depression at the point of puncture. 

 IS days. Line of bacteria has liquefied. 



Agar Plates: — 



Jf8 hours. Colonies are punctiform, 1-1-J mm. in diameter, round, raised, white, 



glistening. 

 With § objective colonies are round, dense in centre, clear margins, granular, 



entire edges. 

 No further change. 



Agar, 37° C.:— 



2Jf hours. Moderate growth, flat, slightly spreading, smooth and translucent. 

 No further change. 



Glucose Agar Slope: — 



Flat, moist, spreading, whitish growth, few gas bubbles. 



No further change except browning of the agar beneath surface. 



Glucose Agar Stick: — 



24 hours. Filiform, growth spreading on surface. 

 48 hours. Few gas bubbles along line of puncture. 

 No further change. 



Beef Broth .•— 



24 hours. Moderate clouding, flocculent, abundant sediment. 



5 days. Clearing. 

 No further change. 



Dunham's Solution: — 



Grown for five days at room temperature, tested with Ehrlich test, allowed to 

 stand 20 minutes and then recorded. Indol positive. 



Litmus MilJc: — 



24 hours. No change. 



48 hours. Slight amount of gas, colour somewhat lighter, no coagulation. 



6 days. Much gas in foam form. No. coagulation. Colour liliaceous. 

 Subsequently milk coagulates, blue ring, surface clear, whey on one side, curd 



adhering to two- thirds of the tube; bleached to a cream colour and of firm 

 consistency. 



