AFFECTED SALMOX, JflRAMICHI HATCHERY 161 



SESSIONAL PAPER No. 38a 



Potato:— 



Slightly raised, moderate growth, cream-yellow. 

 3 iveelcs. Xo further change. 



D. 1. 



Medium-size bacillus, with slightly rounded ends, actively motile, stains well 

 with methylene blue, gram negative. 



Gelatine Plates: — 



Colonies visible to the naked eye in 2U hours. 



JfS hours. Uniform, round, white, glistening colony; § objective round, granular, 



dense to the edge, edges entira 

 S days. Colonies become more dense. Convex. 

 No further change. 



Gelatine SticTc: — 



IfS hours. Line of puncture is villous. Slight softening of the gelatine on the 



surface. 

 Subsequently growth along line of puncture becomes villous to papillate, soften- 

 ing gradually extending along line of puncture. 



Agar Plates: — • 



48 hours. Punctiform to 1 mm. in diameter, round, white, raised, glistening 



colony; § objective colonies round, dense in centre to clear margin, granular, 



edges entire. 

 8 days. Slight increase in sizes; otherwise no change. 



Agar slope, 87° G.:— — 



No growth at this temperature. 



Glucose Agar Slope: — 



Moist, flat, spreading, whitish growth. Agar becomes brown beneath the growth, 

 but no further change. 



Glucose Agar Stick: — 



Line of puncture filiform, spreading on surface, three or four small bubbles 

 appear in 48 hours and slight increase in growth; otherwise no change except 

 browning under growth. 



Beef Broth:— 



24 hours. Growth moderate, sediment moderate and flocculent. 



5 days. Clearing. 

 No further change, 



Dunham's Solution: — 



Grown for five days at room temperature, tested with Ehrlich test, allowed to 

 stand 20 minutes and then recorded. Indol positive. 



Litmus Milk: — 



24 hours. No apparent change, but on tapping the tube small gas bubbles rise 



to the surface. 

 48 hours. Gas more pronounced. Colour liliaceous. 



6 days. Foamy gas. No coagulum. Colour liliaceous. 



8 weeks. Blue ring on surface cleared away along one side, remainder firm curd 

 adhering to the tube. Bleached cream colour. 



