162 DEPARTMENT OF 'THE NAVAL SERYICE 



8 GEORGE V, A. 1918 

 Potato : — 



Moderate growth, raised, rugose, waxy, cream yellow in colour. 



E. 1., E. 2. and E. 3. 

 Resemble A. 1. 



F. 1. 



Medium size bacillus with rounded ends. Actively motile. 

 On staining with methylene blue there are two or three dark granules in most 

 •of the organisms. Gram negative. 



Gelatine plates: — 



£^ hours. Just visible to the naked eye. Round, white, glistening, S object- 

 ive brown, edges entire, granular. 

 Subsequent liquefaction. 



ijtlatine Stick: — 



Growth uniform, line of puncture filiform, growth becomes slightly heavier 

 and on the 6th day there is a slight liquefied depression. 

 10 days. Liquefaction is infundibuliform. 

 13 days. Complete liquefaction. 



Agar Plates : — 

 Agar slope, S7° : — 



Very slight, if any, growth (7 days). 



•Glucose agar slope : — 



Filiform, non-spreading growth. 



-Glucose Stick: — ' 



Filiform growth, nothing on the surface. 

 6 days. Slightly heavier, subsequently no change. 



£eef Broths- 

 Slight clouding, flocculent sediment. 

 3 days, clearing. 

 No further change. 



Jjunham's Solution: — 



Grown for five days at room temperature, tested with Ehrlich test, allowed 

 to stand 20 minutes, and then recorded. Very weak Indol. 



Litmus Milk : — 



6 days. No change visible until 6th day, when colour becomes darker. This 

 increases. 



6 weeks. Colour is atrocyaneus. There is progressive digestion without 

 coagulation. 



Potato : — 



Whitish growth restricted and filiform. 

 3 weeks. No further change. 



G. I. 



Medium size moderately thick bacillus with rounded ends, very considerable 

 variation as to size, actively motile, stains well with methylene blue, gram negative. 



