AFFECTED SALMON, MIRAMICHI HATCHERY 163 



■SESSIONAL PAPER No. 38a 



Gelatine plates: — 



2^ hours. Just visible to the naked eye. 



48 hours. Punctiform. 



8 days. 1-5 mm. in diameter, round, saucer-shaped, liquefaction. Whitish 

 in colour, most dense near centre. Eadiating lines like the spokes of a wheel 

 from the centre consisting of deposited bacteria. With S objective edges are 

 entire and interior granular to grumose. 



4 days. Plates have liquefied. 



■Gelatine Stick: — 



Jf8 hours. Liquefaction heavier, 6 mm. in depth. This increases and is strati' 

 form to sacchate. In ten days tube is completely liquefied. 



Agar Plates: — 



Agar slope. 37° : — 



Very slight growth, in 24 hours. 



48 hours. More abundant growth, spreading, flat, glistening, semi-opaque. 



3 days. Slightly heavier. 



No further change. 



G hi cose agar slope : — 



Moist, white, spreading, smooth. Gas in condensation water. 

 3 weehs. Cream-yellow colour at the base of the slope, and centre of surface 

 growth. 



Glucose SticJc: — 



Filiform, slightly spreading on surface, 3 or 4 gas bubble along line of 

 puncture. 



No further change. 



:Beef Broth: 



24 hours. Strong, cloudy, moderate sediment. 



5 days. Pellicle over entire surface. 



7 days. Yellow-cream colour in the outer layers. 

 No other change. 



.Dunham's Solution: — 



Grown for five days at room temperature, tested with Ehrlish test, allowed to stand 

 20 minutes, and then recorded. Indol very strong production. 



Jjitmus Milk: — 



48 hours. Colour is lighter. 



2 days. Alkaline digestion commences. 



6 days. Almost complete digestion, remaining curd, in fine particles, dirty viola- 



ceous in colour. Whey | of tube. Semi-transparent and avellaneous in colour, 

 no odour. Blue ring at surface. 



Potato : — 



24 hours. No apparent change. 

 48 hours. Slight growth, filiform, yellowish. 



6 days. Moderate growth, slightly raised, moist on the moist part of potato and 

 dry at the top, ferruginious in colour. 



3 weeks. Colour is redder, otherwise no further change. 



