164 DEPARTMENT OF THE NATAL SERYIGE 



8 GEORGE V, A. 1918 



H. I. 



A short to medinm stout bacillus, actively motile, stains well with methylene blue, 

 is gram negative. 



Gelatine Plates : — 



24 liours. Just visible to the naked eye. 

 Jf days. Punctiform, later liquified. 



Gelatine Stick: 



Growth uniform, filiform. 



Jf8 hours. Slightlj^ liquefying, 2 mm. in depth, stratiform. Liquefaction increases 



and is slightly sacchate with flocculence. 

 10 days. Liquefaction becomes infundibuliform. 

 15 days. Whole tube is liquefied. 



Agar Plate: — 



48 hours. Round, uniform, glistening, colony. "With I objective round, edges 



slightly erose. Slightly granular colony. 

 3 days. Colony becomes more massive and bluish white; otherwise no further 



change. 



Agar Slope, 37° :— 



Very slight growth, one or two colonies. Increases along line of puncture. 

 7 days. No further change. 



Glucose Agar Slope: — 



Thin, translucent, moist, film in 24 hours. No further change. 



Glucose Sticlc: — 



Filiform. No surface growth. 



Beef Broth :— 



2If. hours. Slight clouding, flocculent and abundant sediment. 



3 days. Clearing. 



7 days. No further change. 



Dunham's Solution: — 



Grown for five days at room temperature, tested with Ehrlish test, allowed to stand 

 20 minutes, and then recorded. Indol very strong. 



Litmus 2Iilh : — 



2J^ hours. No change. 



48 hours. Tubes become darker in colour, atro-violaceous. No coagulation. 

 Subsequently there is gradual digestion. Whey first with a violet shade, 

 throughout, which gradually concentrates as a deep blue ring on top, and curd 

 becomes semi-transparent, isabellinus in colour, and thick but not viscous. A 

 little undigested curd at bottom of tube. (3 weeks.) 



Potato : — 



24 hours. Yery slight growth. 



Jf. days. Growth moist, slightly raised, smooth. Colour brown, light testaceous. 

 S weeks. Colour changes somewhat between rosaceous and testaceous. No further 

 change. 



