AFFECTED SALMON, MIRAMICHI HATCHERY 



SESSIONAL PAPER No. 38a 



SUMMARY OF CHARACTERS. 



165 



Al 



A 2 



A3 



A 4 



B2 



ei 



Dl 



Ft 



G 1 



HI .... 



B Sal- 

 monis 

 pestis. 



Milk. 



Characteristics of the Micro-organism {Bacillus sahnonis pestis Patterson). 



Morphological Characters. 



A short, thick bacillus with rounded ends, varying in length, occurring singly and 

 in pairs lying end to end. Actively motile, non-spore-bearing, does not stain with 

 Gram's method, grows rapidly and profusely at the room temperature, but shows little 

 or no growth at 37° C, and is killed at this temperature in about six days. 



The organism exposed to a mixture of ice and salt for a week not only survived 

 that low temperature, but grew profusely while in the mixture. Involution forms were 

 only observed in glucose media. It appears to be a strict aerobe. Pathogenic to fish, 

 non-pathogenic to frogs, mice, and guinea-pigs. 



Cultures. — Room Temperature. 



Gelatine Plates. — In about three days small, greyish, pin-point colonies appear, with a 

 ring of liquefaction around them of a transparent greyish colour, which rapidly 

 increases, the plate becoming completely liquefied in about 36 hours after their 

 appearance. The dense pin-point centre and transparent area of liquefaction 

 around is markedly characteristic of the bacillus, together with the very rapid 

 liquefaction of the gelatine. 



Gelatine Stah. — Profuse growth along needle track at the end of 18 hours, which gra- 

 dually increases and rapidly liquefies the gelatine. 



Carhol Gelatine, 1 per cent Stah. — Slight growth in 18 hours along needle track, which 

 gradually liquefies the gelatine. 



Carhol Gelatine, -Oo per cent Stah. — The growth is more profuse. 



Carhol Gelatine, -OS per cent, Stah. — Very profuse growth. 



