166 



DEPARTMENT OF THE XATAL SERVICE 



8 GEORGE V, A. 1918 



Room Temperature. 



Agar Streak : — 



Dense, profuse, cream -coloured moist shining growth along needle track in 

 18 hours, with irregular margin, which gradually spreads over the 

 surface of the agar. 

 Agar Smear : — 



Small pin-i)oint cream-coloured colonies at the end of 18 hours with irregular 

 spreading transparent margins. 

 Agar Glucose Stah : — 



Profuse cream-coloured growth along needle track for about half an inch at 

 the end of 24 hours, spreading on the surface. The agar gradually 

 becomes cloud >i from the surface and parallel to it, and extends for about 

 half an inch down the media. No gas production. 

 Agar Olucosc Plate : — 



Cream-coloured colonies with moist shining surface and white cloudiness 

 around each Colony. 

 Blood Serum : — 

 Bouillon : — 



At the end of 18 hours the bouillon becomes cloudy throughout, with 

 marked skim on the surface and clinging to sides of tube, with a slight 

 deposit at the bottom. 

 Bouillon (Glucose) : — 



Similar to ordinary bouillon, but growth much more profuse 



Bouillon Taurocholate Glucose : — 



Slight growth, turning the media slightly red. No gas formation 



Litmus Milk : — 



In about 48 hours there is a distinct acid reaction, which gradually increases, 

 and in about seven days the milk becomes coagulated and gradually 

 digested. 

 Peptone Water: — 



Marked cloudiness throughout at the end of 18 hours. Gives no indol 

 reaction. 

 Potato: — 



Very profuse yellowish brown growth at the end of 18 hoiirs, raised on the 

 surface of media like blisters, with nioist shining surface. 

 Agar (Anaerobicall'i) : — 



No growth 



37° C. 



(Growth barely visible. 



No perceptible growth. 



Very slight growth. 



No growth. 



No perceptible change. 



Very slight cloudiness at 

 the end of 48 hours. 

 Gives noindol reaction. 



Very slight growth in 48 

 hours. 



No growth. 



The organism also withstands the effect of ordinary water, sterile water and sea- 

 water for a considerable time, as flasks of those inoculated with it and kept at the room 

 temperature for over a month gave profuse growths when reinoculated on agar. It 

 does not, however, survive more than a week in distilled water. It also keeps well ou 

 sub-cultures, as tubes of agar inoculated from sub-cultures about a year old gave pro- 

 fuse growths in about 18 hours. 



The chief characteristics of the hacillus are those : — 



Actively motile, non-spore-bearing bacillus. 



On sub-culture it grows profusely in IS hours at the room temperature. 



On sub-culture it grows profusely when exposed to deg. C. for a week. 



Shows little or no growth at 31° C. 



Is killed at ST C. (98-6° F.) in about six days. 



Liquefies gelatine with extreme rapidity. 



Coagulates and digests milk. 



Forms a cloudiness in gluoose agar in the neighbourhood of the growth. 



Grows well in sea water. 



Strict aerobe. 



Involution forms only observed on glucose media. 



Does not stain with Gram's method. 



Pathogenic to fish. 



Non-pathogenic t« frogs, mice, and guinea-pigs. 



