BACTERIOLOGY OF SARDIXES 187 



SESSIONAL PAPER No. 38a 



obtained from various sources. This in all probability would be due to the 

 glycogen content. While the use of this medium has for some time been dis- 

 continued, I propose to test its value for certain phases of the laboratory 

 analyses. 



Baur^^ in working at Kiel on the denitrifying bacteria used and recom- 

 mends a broth of which mussels are the essential component. 



Beef Peptone Agar. — Standard methods.^^ 



Beef Peptone Gelatine. — Standard methods.^^ 



Glucose Agar. — One per cent glucose added to agar prepared as above, immediately 

 before tubing. 



Loeffler's Blood SerumA- 



Loeffler's Typhoid Solution ^'-K — This medium containing malachite green has been 

 recommended by Loeffler for use in culturing strains of the colon-paraty plioid- 

 typhoid group. 



Aesculin Agar^^. — For specific reaction of organisms of the colon-aero genes group; 

 loops of a broth culture spread on plates. 



MacConJcey's Neutral Red Bile Salt Lactose Broth ^^'. — For reduction test of organ- 

 isms of the colon-aero genes group. 



Bouillon for V oges-ProsJiauer Beaction.^^ 



Bouillon for Methyl Red Reaction.^'^ 



Solution for Reduction of Nitrates to Nitrites '^. — Giltay's synthetic solution was used. 



Dunham Solution for Indol Production.^^ 



Glucose Broth. — One per cent glucose in Dunham solution. 



Fermentation Broths. — For the fermentation reactions I have used ten test substances. 

 It will be seen that in addition to the glucose salicin I have adopted the use 

 of another glucoside aesculin — used in conjunction with iron citrate by Harrison 

 and Vanderleck — as a fermentable test substance in Dunhaih broth. I have 

 been using aesculin for this purpose during the last four months in connection 

 with work on the gas producing organisms in the Ottawa river water, and find 

 a correlation in the black reaction of the aesculin agar medium, and the pro- 

 duction of acid and gas in aesculin used as a carbohydrate test substance. 



Litmus Milh.^^ 



METHODS. 



On account of the comparative paucity in the literature, of descriptions of ,actual 

 methods adopted in the isolation of bacteria from swelled canned fish, the procedure I 

 have followed has largely been determined by experience as the work has progressed. 

 This procedure has been changed as better methods suggested themselves, and in the 

 culturing from the many cans still awaiting examination I propose further changes 

 affecting detail, while the use of additional media which will be to the advantage of 

 the work has suggested itself. 



Isolation of Bacteria from the Cans. 



The oily greasy surface characteristic of the cans with pronounced swelling neces- 

 sitated the use of a disinfecting agent which would disinfect, and remove the oil, at the- 

 38a— 13^ 



