192 DEPARTMENT OF THE XATAL SERVICE 



8 GEORGE V, A. 1918 



To differentiate from the swelled cans obtained direct from the canning factories 

 and from retailers I have designated the salvaged cans as " Swelled cans, series II." 

 The brand of sardines of which this cargo consisted is one of the least expensive brands 

 on the market; cottonseed oil is used. 



Can II. — On shaking, perceptible " rattle " characteristic of the swollen cans. 

 On opening with the cutter escape of gas and pronounced putrefactive odour; 

 contents soft and disintegrated; colour dirty white with tendency to redness 

 in inner portions. 



See Culture 24, Class I; Culture 14, Class H. 



Can III. — 'Characteristic " rattle " ; escape of gas and pronounced putrefactive 

 odour on opening of can; contents soft and disintegrated, and of a dirty white 

 colour. 



See Culture 26, Class I; Ciilture 16, Class II. 



ORGANISMS OF THE GAS-PRODUCING TYPE. 



Culture 2k. 



Source: Can II, Ser. II, Packer A. 



Morphology. — Microscopically : coccus forms to short thick rods twice as long as 

 broad; average lengthl -8 — 1 fx *Gram negative. From old agar cultures no 

 evidence of spores. 



Motility. — In hanging drop occurring singly, in twos and in chains; some individuals 

 with rapid movement, some having slow undulating motion. 



Cultural Characteristics. — 



Agar slope. — 36 hrs. 37° C. — ^growth luxuriant, raised, glistening, iridescent, 

 yellowish-white by transmitted light. 



Loefflers Blood Serum. — 24 hrs., 37 C. moderate, yellowish-white, no liquefaction. 



Loefflers Malachite Green Sol. — Green precipitate or weak coagulum at bottom 

 of tube; this very slowly changes and within 14 days partially digested; 

 liquid portion assuming brownish tint. 



Gelatine Stah. — Koom temperature; liquefaction begins in 24 hrs. crateriform; 

 in three days liquefaction on surface and along track of needle, crateriform 

 to infundibuliform ; growth very slimy on this medium; in 7 days yellowish, 

 cloudy stratiform extending 1 cm. from surface, remainder infundibuliform 

 with heavy yellow flocculent sediment to bottom of tube. In 18 days 

 liquefaction not yet complete; upper portion heavy milky even cloudiness, 

 merging into layers of semi-transparent cloudiness, the lower portion a heavy 

 ferric-yellow mass of precipitate. 



Nxdrient Broth. — 24 hrs. 37°0. — heavy clouding with bluish rim; in 3 days floc- 

 culent flakes of bluish tint on sides of tube; in 5 days very heavy dense even 

 clouding, watered silk appearance; this condition persists. 



Herring Broth. — Condition similar to above; very heavy growth; in 9 days a loop 



of the liquid show'ing decided iridescent bluish sheen. 



Milh. — In 24 hrs. imchanged, except that much froth on shaking; in 3 days 

 coagulated', soft curd, some whey expressed; in 9 days yellow digested fluid 

 2/3 of tube, remainder white soft ciard; in 14 days ropiness noted, and 

 medium almost entirely digested with slight amount of flocculent curd at 

 bottom of tube; in 5 weeks almost wholly turbid yellowish digested fluid with 



slight jelly-like yellowish iridescent flocculent curd on base of tube. 



