194 DEPARTMENT OF THE NATAL SERVICE 



8 GEORGE V, A. 1918 



only after a period of 72 hours. The remaining substances used are fermented 

 moderately well in 24 hours to acid and to gas. 



Glucose. Lactose. Saccharose. Mannite. Dulcite. 



+ + +- ++ + + + - 



Adonit. Eaffinose. Arabinose. Xylose. Salicin. 



++ ++ ++ + + 



Aesculin. Glycerine. Inulin. 

 + + + + + + 



+ = acid. 

 ++ = acid and gas. 

 Culture 26 

 Source: Can III, Ser. II, Packer A. 



Morphology. — Microscopically, rods 11 to 1* times as long as broad; average length 

 1-6 IX with many longer forms even in young cultures. Gram negative* ; from old 

 agar cultures no evidence of spores. 



Microscopic preparations made from cultures of this organism incubated at 

 the same and at different temperatures have shown much variation in morphology ; 

 successive plate culturing, however, has failed to show impurity. 



Motility. — In hanging drop occurring singly, and in twos, sometimes side by side; 

 longer forms noted; non-motile. 



Cultural Characteristics : — 



Agar Slope. — 36 hours, 37° C, moderate, along track of needle, glistening yel- 

 lowish-white by transmitted light. 



Loeffler's Blood Serum. — Growth slight after 72 hours. No liquefaction. 



Loeffler's Malachite Green Solution. — 24 hours, 37°C., coagulated as soft junket- 

 like curd attached to sides and bottom of tube, green, with pale green liquid 

 expressed. After 14 days no change. 



Gelatine Stah. — Room temperature — in 3 days scant- growth, filiform, no lique- 

 faction; in 18 days no change apart from increased growth, no liquefaction. 



Nutrient Broth. — 24 hours, 37° C, moderate clouding, no pellicle, no sediment, 

 no ring; in 3 days watered silk appearance; in 9 days no change except slight 

 sediment at bottom of tube. 



Herring Broth. — Similar to above, but much more luxuriant growth. 



Milk. — In 24 hours at 37° O., no coagulation, much froth on shaking; in 3 days 

 coagulation beginning; in 5 days firm coagulum, no gas, no digestion; in 16 

 day 5 curd slightly split by gas. In 5 weeks shrinking of curd, but no digestion. 



Litmus Milk. — ^In 24 hours violaceus, much froth on shaking, no coagulation; in 

 3 days liliaceous with weak coagulum ; in 5 days curd slightly cracked by gas. 

 In 5 weeks no digestion ; pale lilac to Isabella. 



Aesculin agar. — One loop from peptone broth culture streaked on plates; no 

 reaction. 



Aesculin hroth. — In 24 hours. Slight change but no black reaction; later medium 

 darkened slowly in several days becoming black. 



MacConkey's N.R.B. hroth. — No reduction to canary yellow in 48 hours. 



Gelatine Colonies. — (1st appearance), 72 hours at room temperature. Surface 

 colonies yellowish white by transmitted light, i-l^ mm. diameter; a charac- 

 teristic depression immediately around edge of colony could be seen on tilt- 

 ing the plate; no bluish appearance; no liquefaction. Under the low power 

 objective colonies pale yellow, with paler rim, and entire edges, structure 

 finely granular. 



