BACTERIOLOGT OF SARDINES 



195 



SESSIONAL PAPER No. 38a 



Agar colonies. — 20 hours. 37°C. Growth slow, punctiform, scarcely visible to 

 the eye. Examined 3 days; by transmitted light surface colonies greyish 

 white, elliptical and round, the larger colonies 0-5 mm. diameter. Subsurface 

 colonies similar to above; majority of the colonies immediately under the 

 surface. Under the low power objective all colonies appeared dense, com- 

 pact with edges entire to slightly serrated. 



Temperature Relations : — 



Thermal death point. — 10 minutes exposure in nutrient broth at 60° C. 

 Optimum, temperature. — On agar grows moderately well, room temperature and 



at 37°C. 

 Vitality on Culture Media. — The culture survives several months in artificial 



medium, agar or gelatine. 



Relation to Oxygen. — Incubated for 36 hours under anaerobic conditions, scant growth 

 on glucose agar, small gas bubbles in medium, clouding of condensation water. 

 While growth is noted, the organism prefers aerobic conditions. 



Biochemical reactions: — 



Indol production: Indol not produced. 



Reduction of nitrates : ? 



Voges-Proskauer reaction: Negative. 



Methyl red reaction: Slightly acid. 



Fermentation of Garhohydrates. — The carbohydrates used are but feebly acted 

 upon by this culture. In each case, however — with exception of inulin — 

 those substances which are fermented to gas have shown the positive reaction 

 within 24 hours at 37° C, and no further gas production has taken place even 

 after 5 days. The Andrade indicator has changed to a clear scarlet and no 

 reduction has taken place after prolonged incubation. The two substances 

 most easily acted upon are glucose and saccharose. 



Culture 32. 



Source: — Can. I. Ser. I. Packer B. 



Morphology: — Microscopically short thick rods twice as long as broad; average length 

 1-6 [X staining unevenly with Klihne's methylene blue; some longer and thinner 

 forms, but repeated replating has failed to show impurity. Gram negative*; 

 from old agar cultures no evidence of spores. 



Motility. — In hanging drop occurring singly and in twos, actively motile, progression 

 as in semi-circles. 



Cultural Char'acteristics : — 



Agar Slope. — 36 hours 37° C., luxuriant, raised, thick, along track of needle, glis- 

 tening, iridescent, yellowish white transmitted light, medium slight tendency 

 to brown. 



