196 DEPARTMENT OF THE NAVAL SERVICE 



8 GEORGE V, A. 1918 



Herring agar. — 20 hours 32° C, growth abundant and heavy along track of needle, 

 contoured, yello wish- white ; spreading over slope as bluish film of discrete 

 colonies, transmitted light, glistening, iridescent; heavy clouding condensa- 

 tion water. 



Loeffler's Blood Serum. — 24 hours 37° C, moderate, moist, spreading; no liquefac- 

 tion after 7 days. 



Loeffler's Malachite Green Sol. — Coagulated as soft junket like curd attached to 

 sides and bottom of tube, green, gas bubbles, light green clear fluid expressed ; 

 after 14 days coagulum as precipitation on sides of tube, no reduction of 

 colour. 



Gelatine Stab. — Room temperature — 24 hours filiform, no liquefaction; in 4 days 

 growth abundant; in 1 week no liquefaction and no change in medium, 

 growth equally good in stab and on surface; no liquefaction in 21 ^ays. 



Nutrient troth. — 24 hours 37° C, moderate, clouding, slight pellicle easily dis- 

 lodged, pale bluish rim at top, very slight tendency to flocculency; in 48 

 hours flocculent precipitate suspended and at bottom; in 7 days discrete par- 

 ticles adhering to tube at surface, even clouding, clotted sediment on shaking. 



Herring broth. — Similar to .above, but much heavier. 



Milk. — 18 hours, 37° C, much froth on shaking, no coagulation; in 72 hours 

 coagulation beginning, frothy; in 4 days weak coagulum with whey expressed, 

 gas bubbles, curd splitting, whey white cloudy; in 10 days condition accen- 

 tuated, no liquefaction. 



Litmus milk. — In 18 hours frothy, no coagulation, violaceus, merging into light 

 violaceous near bottom of tube; in 48 hours liliaceous, frothy, no coagulation; 

 in 72 hours still frothy, coagulation beginning; in 4 days coagulated, some 

 whey expressed, curd split by gas holes; in 14 days bleached with red rim at 

 top. 



Aesculin agar. — 1 loop from peptone broth culture streaked on plates. In 24 

 hours 37° C. growth brown-black reaction. 



Aesculin broth. — In 24 hours, black reaction. 



MacConkey's N.R.B. broth. — No reduction to canary yellow in 48 hours. 



Gelatine colonies. — Room temperature (1st appearance). In 72 hours growth 

 luxuriant and rapid; surface colonies up to i — 1 mm. diameter, white and 

 glistening; depression around edge of colony, as if gelatine under tension — 

 See Culture 26. Smaller colonies bluish to white, round; subsurface colonies 

 small, bluish to bluish white. Under low power objective surface colonies 

 dense, pale-yellow, with paler rim and entire edges, structure finely granular; 

 subsurface colonies similar with homogenous structure, round, edges clearly 

 defined and entire. 



Agar colonies. — 20 hours 37° C, growth rapid, abundant, surface colonies 1^ — 

 2 mm. diameter, concave, smooth, glistening, tendency to striate; by trans- 

 mitted light ferric to yellowish-white centre, paling to blue tint at edges, 

 smaller colonies bluish white; subsurface colonies up to -5 mm. diameter, 

 yellowish-white. Under low power objective surface colonies finely granular 

 structure, ferric-yellow paling at edges, edges entire; subsurface colonies 

 dark " mound " appearance in centre, remainder pale lemon, finely granular 

 with tendency to grumose, edges entire. 



Herring agar colonies. — Two to three times diameter of above, umbonate, radiate, 

 concentrically ringed. 



