198 DEPARTMEl^T OF THE NAVAL SERVICE 



8 GEORGE V, A. 1918 



Herring agar. — 20 hours 32 C, growth abundant, contoured, yellowish white 

 growth along track of needle, spreading over slope as bluish film of discrete 

 colonies; glistening, iridescent; heavy clouding of condensation water. 



Ldeffler's Blood Serum. — 24 hours 37° C Moderate, ferric yellow growth, no 

 liquefaction after 7 days. 



Loeffiers Malachite Green Sol. — In 24 hours 37° C coagulated as described in 

 culture 32 ; in 14 days medium assuming a greenish brown tint, no definite 

 reduction and no liquefaction. 



Gelatine stab. — ^Room temperature — 24 hours filiform, no liquefaction, equally 

 good on surface and in stab ; in 4 days growth abundant ; in 7 days no lique- 

 faction and no change in medium; no liquefaction in 21 days. 



Nutrient hroth. — 18 hours 37° C, clouding moderate, slight pellicle; on shaking 

 small flakes perceptible in medium; bluish rim; slight viscid sediment; in 

 72 hours cloudy waves, as watered silk, some flocculent precipitation in sus- 

 pension. 



Herring hroth. — Similar to above, but heavier. 



Milk. — 18 hours 37° O., much froth on shaking, no coagulation; in 48 hours, 

 weak coagulum beginning; in 72 hours coagulated with gas and expulsion 

 of whey, curd later splitting with gas holes. 



Litmus milk. — 18 hours 37° C, much froth on shaking, liliaceous, no coagulation; 



in 48 hours weak coagulation beginning; in 72 hours coagulated, gas, whey 



expressed, later bleaching to Isabella and much splitting of curd by gas. 



Note. — Milks and litmus milks incubated for 2 months have appeared to be 



slowly digesting; up to the present I have been unable to verify this and 



further tests must be made to establish the final condition of the clot. 



Aesculin agar. — 1 loop from peptone broth culture streaked on plate. In 24 hours 

 at 37° C, reaction brown-black. 



Aesculin hroth — In 24 hours black reaction. 



MacConkey's N. B. B. Broth. — In 48 hours, 37° C, slight reduction to eosin tint, 

 but no final reduction to canary yellow. 



Gelatine colonies. — Eoom temperature (1st appearance) surface colonies up to 

 ^ mm. diam. ; by transmitted light bluish- white, glistening, almost transparent, 

 resembling more the description of the B. typhosus colonies than the typical 

 B. Coli colony; flat; subsurface colonies smaller, white to yellow- white, depres- 

 sion around edges, see Culture 32. Under the low power objective surface 

 colonies pale yellow, paling near rim with hedges entire; structure finely 

 granular with clearly defined border around more dense central structure; 

 subsurface colonies similar. 



Agar colonies. — 20 hours 37°C., growth moderate, not so rapid as other cultures; 

 surface colonies 1-1^ mm. diameter, round, concave, glistening; by trans- 

 mitted light bluish with pin-point dark white centre, distinctly radiate. 

 Subsurface colonies dirty white; organism growing better just under surface. 

 Under low power objective surface colonies dark centre, remainder of colony 

 faintly discernible as finely granular lemon yellow, with edges entire; sub- 

 surface dark, compact, too dense for structure to be differentiated, edges 

 entire. 



Temperature Relations : — 



Thermal death point. — 10 minutes exposure in nutrient broth at 60° C. 

 Optimum temperature. — Cultures incubated at room temperature and at 37°C. 



grow well; most satisfactory growth at 37° C. 

 Vitality on Culture medium. — The culture survives several months on artificial 



medium, agar or gelatine. 



