BACTERIOLOGY OF SARDINES 199 



SESSIONAL PAPER No. 38a 



Relation to Oxygen. — The culture is a facultative anaerobe; incubated for 36 hours at 

 37 °C. under anaerobic conditions growth scajit on slope as fine discrete colonies; 

 heavy growth and clouding in condensation water. Slope broken and cracked by 

 gas bubbles, these ^ cm. diameter and extending throughout the medium; much 

 froth. 



Biochemical Reactions : — 



Indol production : Indol not produced. 



Reduction of Nitrates : Kitrates reduced to nitrites. 



Voges-Proskauer reactions : Negative. 



Methyl red reaction : Alkaline. 



Fermentation of Carbohydrates. — The culture has a characteristic action upon 

 duleite; this test substance being fermented profusely to gas in 48 hours; acid 

 and some gas produced within 24 hours. In aesculin, gas appears within 

 48 hours. Inulin is fermented to gas only after 7-10 days incubation. The 

 remaining test substances are fermented moderately well to acid and gas with- 

 in 24 hours; but in no case on further incubation is the reaction profuse as 

 in the fermentation of duleite. 



+ = acid. 

 ++ = acid and gas. 



Culture S5. 

 Source: Can. Ill, Ser. I, Packer A. 



Morphology : Microscopically large coccus forms to short thick rods; -8 fx diam. to 

 1 fx long; stain evenly with Kiihne's methylene blue; Gram negative*; from old 

 agar cultures no evidence of spores. 



Moiility: In hanging drop appearing singly and in twos; no motility. 



Cultural characteristics : — 



Agar Slope. — 36 hours 37° C, moderate to abundant along track of needle, glis- 

 tening, iridescent, porcelain white by transmitted light. 



Herring Agar Slope. — 20 hours 32° C, growth abundant, yellowish white along 

 track of needle raised edges, glistening iridescent, by transmitted light the 

 thinner parts bluish discrete colonies. 



Loeffler's Blood Serum.. — 24 hours 37° C, luxuriant, moist; no liquefaction after 

 7 days. 



LoefPer's Malachite Green Solution : — 24 hours 37°C. Coagulated junket like 

 coagulum clinging to sides of tube, gas; in 72 hours reduced greenish yellow; 

 in 14 days reduced to yellowish-brown slimy looking liquid, partially digested. 



Gelatine Stah . — Room temperature, in 24 hours filiform growth equally good sur- 

 face and stab, no liquefaction, slight gas — presumably from muscle sugar — 

 growth luxuriant. No liquefaction in 21 days. 



Nutrient Broth. — 18 hours 37° C, clouding even, no pellicle, no sediment, bluish 

 rim at surface; in 48 hours heavy clouding, viscid sediment at bottom on 

 shaking; in 72 hours flooculent suspension, later sediment increasing, medium 

 becoming clearer, and flocculency. 



