200 DEPARTMENT OF THE NAVAL SERVICE 



8 GEORGE V, A. 1918 



Herring Broth. — Moderate clouding, bluish rim at surface, pellicle, viscid pre- 

 cipitate on shaking; in 4 days very heavy brown -black sediment, later floc- 

 culency and heavy clouding. 



Mill,-. — In 18 hours at 37° C, frothy but no coagulation; in 72 hours coagulation 

 commencing, gas; in 4 days gas holes in curd, frothy; in 10 days clear whey 

 on surface of soft gassy curd. In 2 months no digestion. 



Litmus Milk. — In 18 hours 37° lilaceus, much froth and gas, no coagulation; in 

 72 hours coagulation beginning; in 10 days tinted whey on surface of soft 

 curd pinkish to Isabella ; no digestion in 2 months. 



Aesculin agar. — 87° O. One loop from peptone broth culture streaked on plates; 

 in 24 hours reaction brown to black. 



Aesculin hroth. — .37°C. Black reaction in 24 hours. 



MacConkey's N.R.B. Broth. — 37°. In 48 hours no reduction to canary yellow. 



Gelatine Colonies. — (Room temperature) (1st appearance). Surface colonies up 

 to ^ mm. diameter, bluish white to white, glistening, smaller colonies more 

 distinctly blue; depression around colonies as noted Culture 32. Subsurface 

 colonies yellowish white, small. Under low power objective the centre yellow- 

 ish brown dense compact surrounded by pale border J diameter of colony; 

 edges entire, clearly defined and hyaline. The differentiation of border from 

 centre bears a close resemblance to colony of Asiatic cholera (plate 227 Kolle 

 & Wassermann Atlas Tafel 10), and is not unlike plate 45 of colon colony 

 (Park & Williams, Path. Micro-organisms, 5th edition, page 110). In the 

 large surface colonies the whole structure is more homogeneous. Subsurface 

 colonies appear similar. 



Agar colonies. — 20 hours 37° C, surface colonies 1^-2 mm. diameter. Flat to 

 umbonate, growth rapid, colony round, surface smooth, glistening, iridescent. 

 By transmitted light ferric-yellow centre paling to bluish at edge. Subsur- 

 face colonies punctiform. Under the low power objective surface colonies are 

 dark in centre, " mound " appearance, gradually merging to pale lemon-brown 

 colour, structure finely granular to grumose; subsurface colonies similar. 



Temperature relations : — 



Thermal Death Point. — 10 minutes exposure in nutrient broth at 60° C. 

 Optimum Temperature. — Cultures incubated at room temperature and at 37°C. 



grow well ; better growth at 37°C. 

 Vitality on Culture Media. — Survives several months, in artificial media, agar 

 or gelatine. 



Belation to oxygen. — Facultative anaerobe; incubated at 37°C. for 36 hours under 

 anaerobic conditions, moderate bluish growth by transmitted light, on glucose 

 agar; spreading over slope as bluish film, small discrete colonies with centre more 

 opaque. Condensation water heavily clouded; much froth; medium throughout 

 tube riddled with gas bubbles. The organism appears to grow equally well 

 aerobically or anaerobically. 



Biochemical Reactions: 



Indol production: Indol not produced. 



Production of nitrates : Nitrates reduced to nitrites. 



Voges-Proskauer reaction: Positive. 



Methyl red reaction: Alkaline. 



Fermentation of Carbohydrates. — The action of the culture on dulcite is vari- 

 able but it evidently is able to ferment this alcohol to gas, some tests being 



