BACTERIOLOGY OF iSARDINES 201 



SESSIONAL PAPER No. 38a 



positive, some negative; the alcohol adonit on the other hand is fermented 

 to acid and profuse gas with frothing in 24 hours. The action on inulin is 

 somewhat characteristic, fermentation to acid and gas with frothing in 24 

 hours; no other strain isolated has such pronounced effect on this test sub- 

 stance. Within 24 hours all the remaining carbohydrates are fermented to 

 acid and profusely to gas with very pronounced frothing. In general this 

 culture is much more active in its fermentation reactions than any of the 

 cultures hitherto described. 



Glucose. Lactose. Saccharose. 



+ + + + + + 



Adonit. RafEinose. Arabinose. 



+ + + + + + 



Aesculin. Glycerine. Inulin. 

 + + + + + + 



+ = acid. 

 -H- = acid and gas. 



Culture 86. 

 Source: Can IV. Ser. I. Packer B. 

 MorpJwlogy. — Microscopially varj-ing from very short stumpy rods to forms twice as 

 long as broad; the majority -8-1 ^ long, staining unevenly with Kiihne's methy- 

 lene blue; Gram negative*; from old agar culture no evidence of spK)res. 



Motility. — In hanging drop occurring singly and in pairs; extremely active motility. 



Cultural Charactenstics : — 



Agar slope. — 36 hours ST'C, moderate along track of needle, glistening iridescent, 



Porcelain to yellowish white by transmitted light. 

 Herring Agar slope. — 20 hours at 32° C, growth moderate, slightly raised, dry 



but glistening, some discrete colonies, by transmitted light blue to yellow. 

 Loeffier's Blood Serum. — 24 hours 37° C, moderate, glistening. No liquefaction 



after 7 days. 

 Loeffler's Malachite Green Sol. — 24 hours 37° C, coagulated as Culture 34, much 



gas; in 72 hours reduced to greenish yellow. In 14 days coagulvmi not 



further reduced but precipitation on sides and bottom of tube; ferric-yellow 



liquid expressed. 



Gelatine stab. — Room temperature — in 24 hours filiform growth equally good on 

 surface and in stab; in 48 hours no liquefaction growth on surface showing, 

 moist; in 4 days growth luxuriant; in 7 days growth becoming brown, medium 

 slightlj tinted; no liquefaction arter 21 days. 



Nutrient Broth. — 18 hours 37° 0., moderate even clouding, no pellicle, bluish rim 

 at top, no sediment; in 48 hours heavy clouding watered silk appearance, 

 later sediment noticeable; no pellicle even after 10 days. 



Herring hroth. — Moderate growth, clouding flocculent suspension, bluish rim, 

 no pellicle; in 48 hours brown viscid sediment precipitated; in 10 days ring 

 on surface, very heavy flocculent growth, black sediment. 



Milk. — 18 hours 37°'C. Much gas on shaking, with froth persistent, no coagula- 

 tion; in 14 days weak coagulum commencing and coagulation slowly com- 

 pleted when examined at the end of two months. 



Litmus Milk. — In 18 hours no coagulation, much froth on shaking with froth 

 persisting; violaceiis merging into heliotrope; no further change in 10 days; 

 in 14 days lilaceus, no coagulation; when examined 6 weeks late coagulation 

 complete, lilacous. 



38a— 14 



