BACTERIOLOGY OF SARDINES 203 



SESSIONAL PAPER No. 38a 



is rapidly decolourized in the aesculin, assuming a lemon yellow tint, such 

 persisting; this colour is partially due to the glucoside itself. 



Source: Can V. Ser. I. Packer B. 



Morphology. — Microscopically rods, three times as long as broad; average length 1-Q jx. 

 Stain evenly. Gram negative*; from old agar cultures no evidence of spores. 



Motility. — In hanging drop occurring singly and in twos; motile; movement varying 

 from revolving motion to a wavelike undulating motion. 



Cultural Characteristics : — 



Agar slope. — 36 hours, 37° C., luxuriant along track of needle, raised, glistening, 

 iridescent, yellowish-white by transmitted light; gas bubbles in medium pre- 

 sumably from muscle sugar in meat extract. At times, particularly in the 

 older cultures, agar growth decidedly slimy, drawing out on the needle. In 

 7 days, medium lemon to brown. 



Herring agar. — 20 hours at 32 °C. Along track of needle heavy, raised, compact, 

 greyish white, glistening, spreading as thick blue-green veil, by transmitted 

 light slightly iridescent, heavy clouding of condensation water. 



Lbeffier's Blood Serum. — 24 hours, 37° C, luxuriant, raised, white, spreading, no 

 liquefaction in 7 days. 



Loeffler's Malachite Green Sol. — In 24 hours precipitated light green coagulum 

 on sides of tube; in 48 hours reduction to yellow beginning in 7 days reduced 

 to yellow and almost entirely digested. 



Gelatine Stah. — Room temperature — in 24 hours liquefaction commencing; in 48 

 hours crateriform to extent of 3mm, continuing down the stab as infundibuli- 

 form; in 7 days liquefaction complete and medium sharply divided into 

 layers; immediately below surface liquefaction appears the colour of turbid 

 whey, in successive layers turbidity and cloudiness gradually disappearing; 

 heavy yellow flaky precipitate at bottom. 



Nutrient Broth. — 18 hours, 37° C, heavy clouding, surface iridescent, pellicle, 

 bluish rim easily detached on shaking — life-belt form — medium slightly floc- 

 culent; in 4 days clouding very heavy, bluish rim; later sediment. 



Herring Broth. — Yery similar to above but heavier growth; in 4 days heavy cloud- 

 ing and thick bluish white pellicle; later flocculent. 



MilJc. — 18 hours, coagulation commencing; in 48 hours coagulated with gas and 

 digestion well advanced; in 10 days more than half digested, whey yellowish, 

 heavy pellicle, soft curd ; in 14 days gas bubbles still persisting, digestion pro- 

 ceeded, I tube, the soft curd adhering to the glass, digestion not proceeding 

 directly from surface to bottom. At a later date when the organism had 



38a— 14i 



