BACTERIOLOGY OF SARDI^^ES 205 



SESSIONAL PAPER No. 38a 



24 hours. It will be seen that of the two glucosides vised, salicin and aescn- 

 lin, the former only is fermented to gas. 



In the later cultural experiments a distinct sliminess appeared in all 

 tubes, in peptone broths with and without added sugars; a pale white rim at 

 surface observed to be slimy after several days at 37° C. 



Glucose. Lactose. Saccharose. Mannite, Dulcite. 



++ +- ++ ++ 



Adonit. Raffinose. Arabinose. Xylose. Salicin. 



++ ++ ++ + + 



Aesculin. Glycerine. Inulin. 

 - + + + + + 



+ = acid. 

 ++ = acid and gas. 



Culture 64- - - 



Source Can. VI., Ser. I, Packer C. 



From this source four strains have been isolated — 64, 64a, 64b aerobically, and 64c 

 anaerobically. The similarity of the strains in culture is such that a detailed descrip- 

 tion of each is not warranted. There are, however, certain cultural differences in 64a, 

 64b and 64c as compared with 64, which I have thought worthy of special mention; 

 and these have been noted in the following description : — 



Morphology. — Microscopically varying from coccus forms to short thick rods; the 

 former '8 fi diameter, the latter li times as long as broad; Gram negative.* The 

 culture has been recently isolated, and no evidence of spores has been obtained; 

 this feature cannot at present be finally reported upon. 



Motility. — In hanging drop occurring singly and in twos; very actively motile; 

 meteoric flashing across the field. 



Cultural Characteristics : — 



Agar Slope. — 24 hours, 37° C., moderate along track of needle, flat, slightly con- 

 toured, edges well defined, iridescent, by transmitted light yellowish white 

 with bluish edges. 



Ldeffler's Blood Serum. — Moderate, no liquefaction; in 72 hours moderate, much 

 less than culture 65. 



Ldeffler's Malachite Green Sol. — 24 houi's 37° C, precipitate at bottom of tube, no 

 coagulum, liquid turbid, pea green colour; in 7 days yellowish brown turbid 

 fluid with ferric precipitate at bottom. 



Gelatine Stab. — Room temperature — 24 hours, filiform, no liquefaction; in 7 days 

 no liquefaction, growth luxuriant, surface and in stab ; yellow growth in stab. 



Nutrient Broth. — 24 hours 37° C, even clouding abundant, 'watered silk" appear- 

 ance, no pellicle, no sediment; in 7 days clouding even, no pellicle, heavy- 

 viscid yellowish white sediment at bottom of tube. 



Milk. — 24 hours 37° C, frothy on shaking, no coagulation; in 72 hovixs soft coa- 

 gulum, much gas, whey expressed, curd shrinking; in 7 days white turbid 

 whey, curd shrinking and split by gas. 



Litmus milh. — In 24 hours liliaceous, much froth on shaking, no coagulation; in 

 72 hours soft coagulum, bleached to isabella, curd perceptibly shrinking; 

 much gas ; in 7 days completely bleached with heliotrope rim at surface, depth 

 of 2 cm. turbid tinted whey, curd rapidly disintegrating and permeated with 

 gas holes. 



N.B. — This culture is violent in its action upon milk. 



