206 DEPARTMENT OF THE NAVAL SERVICE 



8 GEORGE V, A. 1918 



Aesculin agar. — 1 loop peptone broth culture streaked on plates. In 24 hours 37° C, 

 black reaction. 



Aesculin troth. — In 24 hours, 37° C, black reaction. 



MacConhey's N.B.B. Broth. — In 48 hours, 37° C, reduced to canary yellow. 



Gelatine Colonies. — ^Room temperature (1st appearance) — identical with Culture 

 35 — 64a presents some variation. In 72 hours growth rapid abundant, more 

 luxuriant than any of foregoing cultures; surface colonies up to 1 mm. 

 diameter, compact, white, opaque with tendency to capitate, round; the 

 smaller colonies bluish to bluish white. Subsurface colonies small compact. 

 Under the low power objective surface colonies have appearance identical 

 with the literature descriptions of the B. coli colony, edges entire centre dark 

 and opaque; subsurface colonies pale yellow in colour, very finely granular, 

 slightly darker in centre. See Culture 35. 



Agar Colonies. — 20 hours, 37° C, growth rapid, flat, surface colonies 1^-2 J mm. 

 diameter, round with tendency to spread; by transmitted light distinct bluish 

 appearance, glistening, iridescent. Subsurface colonies up to 0-25 mm. 

 diameter bluish to white. Under the low power objective surface colonies 

 have small well defined dark centre, remainder lemon coloured; structure 

 coarsely granular to grumose, edges entire, hyaline and well defined; pale 

 radiate filaments — star-like rays — emanate from the colonies into surround- 

 ing medium. Subsurface colonies dark grumose to " mound-like." 



Agar Colonies 64a. — 20 hours 37° C, growth rapid, surface colonies bluish from 

 1-2 mm. diameter, glistening, iridescent, tendency to run together, forming 

 blue film over agar. Subsurface colonies up to 1 mm. diameter white to 

 yellowish white; some force their way to surface and appear as yellowish- 

 white in centre, spreading on surface to 3 mm. diameter, blue, flat, concen- 

 trically ringed, contoured, edges undulate to lobate. Under the low power 

 objective surface colonies (majority) finely granular at centre to grumose 

 near edge; in some instances characteristic protuberances over agar as in 

 culture 64, edges entire; subsurface lemon yellow, edges entire. 



Temperature Relations : — 



Thermal Death Point. — Exposed in nutrient broth for 10 minutes at 60° C. organ- 

 ism survives; exposed for 10 minutes at 70° C. no subsequent growth; exact 

 temperature not yet definitely determined. 



Optimum Temperature. — Grows well at room temperature and at 37°C. More 

 satisfactory growth at 37° C. 



Vitality on Culture Media. — Not yet determined. 



Relation to Oxygen: — The culture is a facultative anaerobe; incubated for 36 hours 

 under anaerobic conditions at 37°'C. the medium — glucose sugar — is split, riddled 

 with gas bubbles and upper portions blown to top of tube, much froth; heavy 

 cloudy condensation water permeated whole medium. The organism grows with 

 extreme rapidity both aerobically and anaerobically. 



Chemical Reactions : 



Indol production: Indol not produced. 

 Reduction of nitrates: Nitrates reduced to nitrites. 

 Voges-Proskauer reactions : Positive. 

 Methyl red reaction: Alkaline. 



Fermentation of Carbohydrates. — -The culture fails to ferment dulcite and adonit 

 to acid or gas. All other test substances used are fermented within 24 hours 



