BACTERIOLOGY OF SARDINES 21 1 



SESSIONAL PAPER No. 38a 



Pronouuced clouding of the media by each inoculum was by that time evident. Plates 

 were made on glucose agar, and after incubation at 37°C. for 2i hours, typical colonies 

 were picked off and streaked on agar slopes. Subsequently series of inoculations were 

 made, and the organisms isolated proved to be identical respectively with the strains 

 with which the experimental cans were inoculated. 



Cultures 35, 37 and 64 respectively have experimentally produced typical swelled 

 cans, have been re-isolated and proved culturally identical with the original strain. 

 The " Postulates of Koch " have been satisfied. 



ORGANISMS WHICH DO NOT PRODUCE GAS. 



Culture 7. 



Source: Herring Excreta. 



Morphology: — Spore forming rods, occurring singly, in twos and in long forms. 

 Gram negative. 



Cultural Characteristics : — 



Nutrient broth. — In 24 hours at 37°C., membranous pellicle, medium clear; 



1 month yellow sediment, medium clear. 

 Alilk. — In 5 days pellicle, no change; in 1 month yellow turbid digestion extending 



I down tube. 

 Litmus Milk. — In 24 hours no change; in 10 days pellicle, sediment, digestion 



with colour varying from yellow to dark purple. 

 Gelatine Stah. — Room temperature, liquefaction beginning in 2 days. In 5 days 



napiform to a depth of 5 mm., remainder filiform; in 14 days liquefaction 



still proceeding with lower part of stab a discrete villous growth; medium 



ferric lemon. 



Biochemical Reactions : — 



Indol: not produced. 

 Nitrates : not reduced- 

 Glucose broth : acid, even clouding, no gas. 

 This culture in its reactions is typical of many strains isolated from herring 



excreta. 



Culture 21. 



Source : Normal Can sardines. Packer A. 



Morphology. — Extremely long thin rods, forming spores; in hanging drop occurring 

 singly and in twos, motile. Gram positive. 



Cultural Characteristics: — 



Nutrient broth. — In 24 hours at 37°C., slight clouding, no pellicle; in three days 

 membranous cup-shaped pellicle, medium cloudy; later, pellicle luxuriant, 

 thick creamy, medium yellowish brown. 



Milk. — No change up to 5 days, when weak coagulum beginning; in 9 days tubes 

 half cogulated; in 16 days yellow digestion nearly complete, remainder of 

 medium firm hard curd. 



Litmus Milk. — No change in 24 hours; in 3 days pellicle, upper layers of milk 

 dark purple, remainder violaceus, no coagulation; in 9 days digested with- 

 out previous coagulation to muddy looking yellowish brown liquid. 



Ldefjfers Blood Serum: — Rapid liquefaction. 



