212 DEPARTMENT OF THE NATAL SERVICE 



8 GEORGE V, A. 1918 



Gelatine Stah: — Eoom temperature, in 24 hours crateriform liquefaction beginn- 

 ing; proceeding slowly in 7 days to 5 cm. from surface of stab; in 18 days 

 not complete, layers of yellowish precipitate. 



Biochemical Reactions : — 



Indol: not produced. 



Nitrates: not reduced. 



Glucose broth : acid, chiefly at surface, no gas. 

 From the same can, and other normal cans, strains were isolated which according 

 to the reactions noted proved to be identical with this culture. 



Culture IS. 

 Source: Swelled Can I, Series II, Packer A. 

 Morphology. — Large coccus, occurring as staphylococcus, no spores, Gram positive. 



Cultural Characteristics : — 



Nutrient broth. — 24 hours at 37°C., moderate, cloudy; no pellicle. 



Milk. — In 5 days no change; no change in 1 month. 



Litmus milk. — As milk. 



Gelatine Stah. — Room temperature. In 2 days no liquefaction ; in 5 days scant 

 growth filiform to discrete; in 14 days medium faintly browned, growth in 

 stab discrete and ferric yellow tint; no liquefaction, growth better under 

 surface. 



Biochemical Reactions: — 



Indol: not produced. 



Nitrates : ? 



Glucose broth: Acid, even clouding, no gas. 



Culture 28. 

 Source: Same Can as Culture 13. 



Morphology. — ^Long rods many times longer than broad, oval spores formed; Gram 

 negative; in hanging drop appear singly; in twos and in long chains; motile 

 with fflidins: movement. 



Cultural Characteristics : — 



Nutrient Broth. — 24 hours 37° C. Moderate, cloudy, slight pellicle; in 1 month 

 cloudy with flocculent yellow sediment. 



Milk. — In 5 days no change; in 1 month digested completely, yellow turbid fluid. 



Litmus Milk. — In 10 days dark purple fluid with no previous coagulation; un- 

 changed in 1 month. 



Loeffler's Blood Serum. — Rapid liquefaction. 



Gelatine Stah. — Room temperature, in 2 days slight liquefaction noted; in 5 days 

 liquefaction progressed to depth of 2 mm., stratiform, remainder of stab dis- 

 crete colonies ; in 14 days liquefaction 1 cm. depth, stratiform yellowish layers 



Biochemical reactions: — 



Indol : not produced. 

 Nitrates: not reduced. 



Glucose broth: Acid, upper part, pellicle, no gas. 

 Cultures 13 and 2^ typical of several strains isolated from such cans. 



