BACTERIOLOGY OF SARDINES 



213 



SESSIONAL PAPER No. 38a 



Culture llf.. 



Source: Swelled Can II, Series II, Packer A. 



Morphology: Long rods many times longer than broad; spores. Gram positive; in 

 hanging drop occurring singly, in twos, and in chains; appear at first immobile 

 but prolonged examination reveals slow laboured movement, some individuals 

 appearing to push themselves along. 



Cultural Characteristics : — 



Nutrient "broth. — 24 hours 37° C, cloudy, flocculent pellicle; in 10 days heavy 

 clouding with some flocculency; in 1 month clouding and yellow precipitate 

 at bottom of tube. 



Milk. — In 5 days no coagulation, ring, pellicle; in 1 month coagulated, some 

 yellow whey expressed. 



Litmus milk. — In 24 hours no change; in 10 days lilac, no coagulation; in 1 month 

 coagulation, and some whey expressed. 



Gelatine Stah. — Eoom temperature — in 2 days moderate growth, dip in gelatine; 

 in 5 days crateriform liquefaction and spreading growth on surface of stab; 

 in 14 days liquefection varied from V-shaped to crateriform to depth of 1 cm., 

 cloudy; remainder of stab discrete. 



Biochemical reactions: — 



Indol: not produced. 



Nitrates: not reduced. 



Glucose broth : acid, more particularly near the surface, no gas. 



