222 DEPARTMENT OF THE NAVAL SERVICE 



8 GEORGE V, A. 1918 

 Motility. — Eapid movement, darting to and fro, many revolve as on an axis. 

 Cultural Characteristics: 



Agar Slope.— 24, hours at 37 °C.— moderate, bluish by transmitted light, moist, 

 glistening, slightly raised, later becoming by transmitted light yellowish in 

 centre gradually merging into transparency. 



Glucose Agar Slope. — Gas, growth moderate to luxuriant, glistening, slightly 

 raised. 



Tochtermanns Serum Agar Slope. — Moist, slightly raised, bluish by transmitted 

 light, spreading discrete colonies, gas. In 8 days growth had become yellow, 

 much water of condensation, heavy greyish-white precipitate at base of slope. 



Loefflers Blood Serum. — Moderate, filiform, moist, glistening, no liquefaction, 

 no discolouration. In 7 days no digestion, no discolouration. 



Sodium Sulphate Agar Slope. — Raised, spreading, moist, no reduction. In 8 days 

 no reduction. 



Gelatine Stah. — 21°C., 24 hours, growth filiform, equal surface and stab; 7 days, 

 tendency to echinulate. In 6 weeks no liquefaction, growth yellowish-brown; 

 characteristic lateral growths resembling a poplar tree against the horizon; 

 medium unchanged. 



Nutrient Broth. — 37 °C. 24 hours. Clouding abundant, no pellicle, no sediment, 

 bluish rim at top. In 1 week, slight sediment; otherwise no change. 



Potato. — Moderate, flat, yellowish-white along track of needle. 



Milk. — In 6 weeks no change. 



Litmus Milk. — Varies from no change to a tint slightly more alkaline than con- 

 trol; blue rim at top. 



Aesculin Agar. — Black reaction, growth less luxuriant than in Culture I. 



Neutral Red Bilesalt Agar. Moderate, pink reaction. 



Peptone Broth + Aesculin. — ^Black reaction. 



Gelatine Colonies.— 5 days at 21°C. — colonies up to 5 mm. diameter; under low 

 power objective granular; edges lobular to contoured, centre dark with paling 

 towards edges. Deep surface colonies granular centre with dark concentric 

 rings. 



Agar Colonies. — 24 hours at 37° C- — surface 1 mm. diameter, raised, concave, bluish 

 by transmitted light, round, smooth, edges entire. Under low power objective 

 granular, edges entire. 



Temperature Relations: — 



Thermal death point: 10 minutes exposure in nutrient broth at 55°C. 



Optimum temperature: 37°C. ; cultures incubated at 37°C., 21°C. and 14°C. 

 respectively. 



Vitality on Culture Media: — 



Active cultures have been recovered from agar tubes after 5 months at tempera- 

 ture of 15°-20°C. 



Relation to Oxygen: — 



Facultative anaerobe; glucose agar. 



Biochemical reactions: — 



Indol production : Indol not produced. 



Reduction of nitrates : Nitrates reduced to nitrites. 



Voges-Proslcauer reaction: Positive, after 6 hours. 



Methyl red reaction: Faint acidity, shortly followed by reversion to alkalinity. 



