BACTERIAL DESTRUCTIO^^ OF COPEPODS 227 



SESSIONAL PAPER No. 38a 



4. Based on their cultural features and biochemical reactions the organisms are 

 classified as follows: — 



Culture I. — Tentatively as a non-motile strain of the sub-type B. communior 

 (Durham) of the type B. coli (Escherich) ; or to take the differentiation further, 

 as B. neapolitanus, a sub-type of B. coli (Escherich). 



Culture II. — Considered tentatively as an atypical form of the Para- 

 Gaertner group after Savage. 



Culture III.- — Identical with M. zymogenes and placed as a variety of the 

 type of liquefying streptococci, Streptococcus gracilis. 



5. No inoculations of these cultures have been made into healthy copepods owing 

 to distance from the sea. 



6. It is not legitimate to draw any definite conclusions regarding the relationship 

 of these organisms to the destruction of the copepods, as no inoculation experiments 

 have been carried out, and the postulates of Koch have not yet been satisfied. Accord- 

 ing to the descriptions presented, however, the evidence is strong in favour of Culture 

 III being a possible causal agent. 



I wish to thank very cordially Dr. F. C. Harrison for his kindness in reading the 

 proofs, and particularly for his valuable and critical assistance with regard to the 

 classification of the B. coli group; and Dr. Arthur Willey for the initial suggestion 

 that I should undertake the investigation. 



EEFEEENCES. 



1. American Public Health Association, 1915 — '" Standard Methods Water 

 Analysis," 77-137. 



2. Besson, 1913— '" Text Book Practical Bact., etc.," (Longmans), 53. 



3. Besson, 1913 — "Text Book Practical Bact., etc.," (Longmans), 52. 



4. Harrison and Yanderleck, 1908 — " Aesculin Bile Salt Agar for Water and 

 Milk Analysis," Trans. Eoy. Soc. Can. III. Ser. II, 105-110. 



5. Savage, 1906 — " Bacteriological Examination of Water Supplies," London, 221. 



6. Voges and Proskauer, 1898—" Zeit. fur Hyg." 28, 20. 



6. Harden, 1905, 1906 — " On the Voges-Proskauer Eeaction for Certain Bacteria," 

 Proc. Eoy. Soc, 77, 424. 



6. Levine, Max., 1916 — " The Significance of the Voges-Proskauer Eeaction," 

 Jour. Bacteriology I, 153-164. 



6. Clark and Lubs, 1915 — '' Differentiation of Bacteria of the Colon- Aerogenes 

 Family by Indicators." Jour. Infectious Diseases, 17, 160-173. 



6. Levine — " Correlation of the Voges-Proskauer and Methyl-red Eeactions on 

 the Colon-Aerogenes group." Jour. Infec. Diseases, 18, 368-367. 



6. Levine — Private Communication. 



7. Clarke and Lubs, 1915 — " Differentiation of Bacteria of the Colon-Aerogenes 

 Group." Jour. Infectious Diseases, 17, 160-173. 



8. Bohme, 1905— Centrall. fur Bakt, Abt. I, Orig. XL, 129-133. 



9. MacConkey, 1909— Journal of Hygiene, 9, p. 91. 



9. Hollman, 1914 — " Decolorized Acid Fuchsin as an Acid Indicator in Carbo- 

 hydrate Fermentations." Journ. Infec. Dis., 15, 227-233. 



