PHYSIOLOGICAL INFLUENCE OF OZONE—HILL AND FLACK. 623 
Fat has a power of absorbing ozone until it smells strongly of 
ozone, and it retains the ozone for along time. Mr. F. Kidd kindly 
tried for us the application of ozonized lard or vaseline to foul chronic 
ulcers of the leg, but found that while hot fomentations were effica- 
cious in cleaning up and rendering sweet control cases of ulcer, the 
ozonized ointment had little effect. 
For the investigation of the respiratory metabolism we used the 
Haldane-Pembrey gravimetric method.. Mice or small rats were 
placed in a beaker fitted with a thermometer and the beaker placed 
m a Hearson air bath to keep the external temperature constant, 
In a first series of experiments the ozone was generated by a specially 
made small ozonizer, and led partly through the animal chamber 
and partly through a collecting wash bottle, in order that its con- 
centration might be determined. 
The water vapor given off by the animal varied so much with the 
passing of urine and feces, and possibly with the animal putting up 
its fur, as it does when depressed by the ozone, that we can lay no 
weight on the calculation of oxygen intake. The weakness of the 
gravimetric method lies in the fact that the oxygen is calculated 
from the difference between loss of body weight and output of water 
and ©O,, and not directly measured. 
We shall confine our considerations in these experiments to the 
loss of body weight, and the CO, output. Table II shows the loss 
-of weight sustained by the animal, the amounts of H,O and CO, 
given off before, during, and after ozone. It is seen that there is a 
marked depression during and after the administration of ozone. 
- The table gives a random selection made from 25 similar experiments. 
The figures for the loss of weight represent the loss of weight of 
the animals weighed in the respiration chamber. Evaporation of 
urine and feces when passed contribute to this loss. As the loss of 
body weight is also influenced by the passing and evaporation of 
the urine and water from the feces, the CO, output results are the 
more trustworthy. 
As the concentration of ozone given by the small generator seemed 
to be too high, we obtained the ozone in the following experiments 
by generating it in a room, the ozonizer being placed at distances 
varying from 100 to 350 cm. from the inlet of the ventilation current 
which was drawn through the animal chamber. Table III gives the 
loss of weight and amount of CO, given off in milligrammes in the 
last eight experiments made under these conditions, the reading for 
the oxygen as before being variable. In all, 16 similar experiments 
were made on small rats. 
