PHYSIOLOGICAL INFLUENCE OF OZONE—HILL AND FLACK. 625 
TABLE III. 
Rat. Air, Air, Air, Ozone, First Second Third | Fourth 
} hour. | } hour. | 4 hour. | 4 hour. (after (after (after (after 
“ 4 4 hour).| 3 hour).| 3 hour). |4 hour). 
—_——_— = | 
A....| Loss of weight....._.. 102 TOSS yee 90 88 74 | as 
COz given off......._. 132 135. | Petes ee 73 132 129") Us 4 Tuy 
B....| Loss of weight........ 119 130a}5 2. eY 7 88 144 | 200 er 
COzg given off....._... 104 1CAUM ees ee 130 127 156 108: |. Pe. 
A....| Loss of weight........ 113 122" | see eee 63 57 65 75) sc See Be 
COz given off......... 91 2: Nal (ie es 86 83 100 | Bl Ee, Settee 
Ce Loss of weight........ 168 Toa): Sera. 190 235 191 122 145 
COs given off........- 173 NU 2 Pe oe ees 199 187 182 140 155 
D....| Loss of weight. ....... 107 104 103 136 80 | 97 128 95 
COz given off......... 142 130 129 155 127 | 130 132 128 
E....] Loss of weight....__.. 295 230 215 145 185 | 123 902) | rR ee 
COz given off. ........ 132 135 114 91 97 77 lt) S| See 
KS: --1 Loss of weight... -.- 145 270 225 235 215 235 1655 | oo 
COz given off........- 163 177 162 165 145 137 LOZ" cena 
G....} Loss of weight....._.. 115 195 197 148 177 145 109 1149 
255 
COs given off......... 153 188 200 163 205 173 175 1110 
138 
Wepre beast apes er represents the result of a further 14 hour of ozone, and the lower numbers that 
The subject was provided with a mouthpiece, fitted with inspiratory 
and expiratory valves. (We used the excellent mica valves made by 
Messrs. Siebe, Gorman & Co., and used in their mine rescue appa- 
ratus.) While inspiring atmospheric air, the subject expired into a 
large canvas-rubber bag of suitable construction, and previously 
emptied of air. After a period of 10 minutes a fresh bag was sub- 
stituted, and the volume of expired air in the first bag was measured 
by pressing the contents of the bag through the meter, and a sample 
of the expired air was collected and analyzed. Successive samples 
- were thus taken, some when the air was ozonized and some when it 
was not. The composition of the atmospheric air being known, the 
requisite data were calculated from the measurements of the meter 
and the analysis of the samples, all results being reduced to 0° C. and 
760 mm. 
In all we have made 19 experiments, and append the details of 
the last 7. In the preliminary trials of the method we found con- 
siderable variations in the metabolism in successive periods of time. 
These were due to want of complete rest on the part of the subject. 
(See Table IV.) 
Our last series of experiments were carried out with the subject 
recumbent on a couch and prepared for the test by a preliminary 
period of rest. Even then, the opening and shutting of the windows 
38734°—sm 1911——40 
