440 TRANSACTIONS OF THE CANADIAN INSTITUTE. Vir vas 
cell as it is now found does not represent what it once was, that it is the 
product of the degradation of a higher form of cell. In that case it may 
be contended that one cannot obtain from the present structure of the 
cell data which can be of service in ascertaining what the structure of 
the primal cell was. Ifthe yeast cell, which, as I claim, is non-nucleated, 
is the product of the degeneration of a higher type of cell, of, for 
example, a nucleated cell, an examination of it may still serve a 
purpose in assisting in determining the origin of the cell nucleus, for we 
can then ascertain how in the yeast cell its surplus of chromatin is 
distributed in the cytoplasm in all conditions, and this may possibly 
indicate to us in what manner the original non-nucleated cell disposed 
of the excess of the analogous chromatin compound. 
From this point of view, therefore, the employment, not only of 
methods of staining, but also and particularly of micro-chemical 
processes in the investigation of these low forms of life is absolutely 
necessary if results of any value are to be obtained. These methods I 
have endeavoured to employ to a certain extent and this constitutes the 
excuse for adding one more contribution on the subject. 
CONTENTS. 
I.—THE CYANOPHYCE. PAGE. 
Miteraturevacyveteierelcseks cio ne terials oh alsiare teeters -)etienelere ener ache Tee Rist He sath apace 441 
Methods of'Study and Material Employed: 235 20 -scse os oo erie 452 
The Living Gellinithe!\Gyanophy cezeeacee ance oe eka ee eee eee 455 
Fixed Preparations: of the Gyanophycecely. e245 1. Soe oe aie = eee 457 
The!Granulesunithe Cyanophy.ceze ses ee-- ieee sie cithace dosent 465 
HM NoVM EAT corn Cuan anne ee uootaoDe co pood bees emdedos blo S.dbo ores oaad.conco ec 469 
Cell Division scence ee eteicst Oe aie ieee sion olero cre isnt cine ais ere eee rea 470 
SUC hein une raorae Ror has sah ncha ad om UA are Too Obod. code konsco cece 472 
II.—BEGGIATOA. 
Miterature iy sessrecr sone cseyece versie ters os tare tare one, eheecece Se fel late lc leret aero Tater ot eal 474 
Methods of studyand) Horms Studied titi. s-c - oa micas ste) aie oie laraielorstctat terete 476 
General: Cell'Stracture™, x15 niece. eile avelents oie akctele telecon cio oetert ner eens 477 
Sumamansyis ic 5, dpic.oie esse aie let aoe et olainayess alo Mists oteTe cis ore mae heve one miedo ioletolel ie yerteriettars 480 
I1].—THE YEAST CELL. 
LBs (itsiosh ible ee ar ae A AON AA NRA RS A Wh ARG rre holo oe Pc euCrathC Caron. tacos 481 
Methods of Study, and Species|Studied\ 23.5. - epee) scenes oe yale eer 488 
General Gell’S tp ture raptor reco iach ora lotel otserru ise loiehel ave lateKcle\olowarcietet eta 490 
The Chromatin-holding Structures) >. .¢).-).5..20 2k 22... 6. SAfiaSeie Hankahe teres Seats 492 
Budding) aad! Spomilationzaa-s a oy ee io eel eee 498 
