1898-99. | ON THE CYTOLOGY OF NON-NUCLEATED ORGANISMS, 447 
latter fact suggesting that the chromatophore is formed of an exceed- 
ingly delicate reticulum. The granules, according to Zukal, are nuclei, 
-and as such divide, the divisions not being followed necessarily by 
‘division of the containing cell. 
Hieronymus! found a thin hyaline layer enveloping the cellular pro- 
toplasm, and applied to the internal surface of the cell membrane. The 
chromatophore is, according to his view, of fibrillar structure, and on it 
are arranged fine granules which appear to contain the chlorophyll. 
The phycocyan is dissolved in enchylema. The central body is formed 
of a wound fibril, and contains all the remaining granular elements, 
which he regards as crystals of the regular system. The substance of 
these crystals he calls cyanophycin, and though he recognizes that it 
does not correspond in its character to nuclein, yet he looks upon it as 
related to the chromatin and pyrenin of the higher organisms. The 
central body is, further, an open nucleus, that is, a nucleus without a 
membrane. 
In reply to Zacharias’ who criticized his observations, Hieronymus? 
‘maintains that there is only one kind of granules in the cells of Cyano- 
phycez, and that two kinds appear to be present because of the methods 
of preparation. He found that when the fixed cells were treated with 
ammonia vapour all the granules received a dark blue colour from 
hamatoxylin, and that all the granules dissolved in diluted acid solutions, 
although they varied in the readiness with which they dissolved. 
According to Palla‘ the cell in the Cyanophycee consists of a colour- 
less “central body,” a coloured peripheral layer, the chromatophore, an 
external investing colourless layer, and possibly also a colourless zone 
(Plasmaschicht) situated between the chromatophore and the “central 
body.” The “central body ” reacts with dyes like a nucleus, but it con- 
tains no granules, and is homogeneous. Its division takes place through 
constriction. The granules in the cell are of two kinds: one composed 
of a substance, cyanophycin, soluble in dilute hydrochloric acid, and 
which stains pure blue with hamatoxylin, found usually in the extreme 
periphery of the chromatophore, and representing the first assimilation 
product of the latter; the other, composed of a viscous substance, 
insoluble in dilute acids, and staining reddish-violet with hamatoxylin. 
1 “* Beitrage zur Morphologie und Biologie der Algen,” Cohn’s Beitrage zur Biologie der Pflanzen, Vol. 
V, p, 461, 1892. 
2 ‘* Ueber die Zellen der Cyanophyceen,” Bot, Zeit., No. 38, 1892, and No. 15, 1893. 
3 *‘Ueber die Organization der Phycochromaceenzellen,” Bot. Zeit., 1893, p. 73. 
4 ‘“‘Beitrage zur Kentniss des Baues des Cyanophyceen-Protoplasts,” Jahrb. fiir Wiss. Bot., Vol. 
XXV, p. 511. 
