448 TRANSACTIONS OF THE CANADIAN INSTITUTE. [VoL. VI. 
The second class of granules, which he identifies with the “Schleim- 
kugeln” of Schmitz, are found in the immediate vicinity of the “central 
body,” but never zz this structure, and rarely only in the chromatophore. 
They are identical with the “ nucleolus,’ the “central substance,” and the 
“red” granules of earlier observers, but their significance is unknown to 
Palla. He calls attention to the fact that in the living cell they are 
stained with methylene blue, which leaves unaffected peripherally placed 
granules, formed of the “cyanophycin.” The chromatophore has a 
vesiculated structure, and the vesicular walls are free from colouring 
matter, which is connected with numerous small granules placed in (?) 
the vesicles. 
In 1894 Fischer’ reiterated his objections to Biitschli’s results, basing 
these upon more extended studies of the structure of Bacteria. 
Nadson’s observations were carried out upon a large number of forms 
fixed and stained in various ways. He found that the coloured 
peripheral zone of the cell is not a chromatophore but that it is proto- 
plasm containing phycochrome. This peripheral layer is vesiculated in 
the sense of Biitschli’s “Wabenbau.” The vesicles are filled with a plasma- 
tic substance which is physically rather than chemically distinguishable 
from that forming the walls of the vesicles. Chlorophyll and phycocyan 
are, however, only in the walls. The central body is sharply differentiated 
from the coloured zone and contains vesicles, but these are less readily 
observable than those found in the peripheral layer, and they are filled 
with a strongly stainable substance. Two kinds of granules are present. 
Of these, one consists of those called. “red” by Biitschli, or the 
“ Schleimkugeln” of Palla and Schmidt, and according to Nadson are 
composed of a substance closely corresponding to chromatin. Such 
granules occur chiefly in the central body and in the walls of the vesicles, 
and their number in a cell varies very much, but whether many or few 
are present the cell is equally capable of division. The second kind of 
granules, called by Nadson, the reserve granules, correspond to the 
“cyanophycin ” granules of Borzi and Palla, and are composed of a sub- 
stance comparable to the starch of the Chlorophycee. They occur only 
in the coloured zone, particularly in the neighbourhood of the transverse 
walls. They colour with hematoxylin blue-violet like the protoplasm 
of the peripheral layer. 
Nadson found a third kind of granules, probably of a plasmatic 
1 ‘‘ Untersuchungen iiber Bakterien,” Jahrbuch fiir Wiss. Bot,, Vol. X XVII, 1894. 
2 ‘‘ Ueber den Bau des Cyanophyceen-Protoplastes,” (In Russian with resumé in German), Scripta 
Botanica, Vol. IV, St. Petersburg, 1895. I have not, unfortunately, access to this publication and have 
had to rely on the reference given in Botanischer Centralbl. Vol. LXIII. p. 238. 
