1898-99. } ON THE CYTOLOGY OF NON-NUCLEATED ORGANISMS. 449 
nature, in the nodal points of the protoplasm in the peripheral zone. In 
Merismopedia and Aphanocapsa the “chromatin” granules in division 
arrange themselves in the form of a figure 8, which after division falls 
into two circles. 
The central body, in Nadson’s view, is similar in many respects to the 
nucleus of higher organisms, and doubtless corresponds to the same. It 
may be regarded as the forerunner of the latter, but he doubts if all cell 
nuclei are derived from it. 
Macallum’ found that the stainable granules in the central body do 
not dissolve when submitted to artificial gastric digestion, but they 
disappear after treatment with a solution of potassic hydrate of 0.1 per 
cent. strength for twenty-four hours. This treatment also deprives the 
central body of its capacity of fixing colours in itself. These facts were 
held to indicate that a nuclein-like substance is present in the central 
body and in its granules, and this view was confirmed by the demon- 
stration of the presence, in these parts, of “masked” iron. The “cyano- 
phycin” or “reserve” granules, which stain specially with picrocarmine, 
may not contain “masked” iron and dissolve readily in dilute 
solutions of hydrochloric acid. The substance forming these granules 
was found to be unlike that constituting the granules of the central body, 
which was held to be in many respects like chromatin. 
Biitschli’s’ third contribution on the structure of the Cyanophycee 
appeared in the next year and in this he defends the views which he 
advanced in his earlier publications on the subject, and criticizes the views 
and observations of Deinega, Hieronymus, Palla, Nadson and Fischer, In 
answer to the latter’s objections he denies that the peripheral layer in 
the cells of the Cyanophycee and Bacteria in his preparations is due to 
plasmolysis and shows that in some at least of the blue-green forms the 
existence of a peripheral zone and a central body can be determined in 
the living cell. In these forms, and especially in some Osczllaria, the 
two parts may be set free through rupture of the cell membranes, and 
then one can distinguish both parts as very distinct. Biitschli strives 
particularly to show that the vesiculated (Waben) type of structure pre- 
vails in both parts and in the two classes of organisms and for this 
purpose gives photographs of living forms in which this structure 
was distinctly demonstrated. He found also that the colouring 
matter of the peripheral zone appeared to be dissolved in the sub- 
1 *‘On the Distribution of Assimilated Iron Compounds, other than Haemoglobin and Hzmatins, in 
Animal or Vegetable Cells,” Quart. Journ. Micro. Sci., Vol. XX XVIII, p. 175, 1895. 
2 ‘Weitere Austiihrungen iiber den Bau der Cyanophyceen und Bacterien,” Leipzig, 1896, 
