450 TRANSACTIONS OF THE CANADIAN INSTITUTE, [VoL. VI. 
stance forming the walls of the vesicles (Waben) and not in the 
contents of the latter. 
Amongst the points dealt with by Biitschli were those relating to the 
nature of the granules and to the homology of the central body. As in 
his earlier observations he found two kinds of granules; one staining 
red-violet with Delafield’s hematoxylin and called on this account 
“red” granules; the other, in Oscz//avz@ usually adjacent to the transverse 
septa and consequently in the peripheral layer, unaffected by hama- 
toxylin but coloured red with eosin and called, after Nadson, “ reserve” 
granules, or, after Borzi, “cyanophycin” granules. The “red” granules 
are, at times, as Hieronymus and Palla found, hollow bodies. They 
occur in both parts of the cell but they are chiefly found on or in the 
outer portions of the central body imbedded in the nodal points of its 
vesicles. This observation is opposed to the views of Zacharias and 
Palla, who maintain that the central body is free from granules. The 
substance forming them Biitschli believes to be chromatin, and the 
evidence in support of this view he finds in the staining properties of 
the granules. Hzematoxylin usually does not stain chromatin red or 
red-violet, the exceptions being the granules formed of this substance in 
the nuclei of Euglena and Diatoms, but the granules in the cytoplasm 
of the latter and in some Protozoa give a similar red stain, a fact which 
tells against “red” granules in Cyanophycee being considered as com- 
posed of chromatin. Biitschli, however, found that in the living forms 
the cytoplasmic granules which stain red with hematoxylin colour red 
with methylene blue, but the nuclear granules in Euglena and Diatoms 
are rendered blue with this reagent. Now Lauterborn discovered that 
in living Oscellarie methylene blue also gives a blue colour to the 
granules which, in hardened preparations, stain red-violet with hama- 
toxylin. These facts, Biitschli thinks, indicate that the “red” granules 
are formed of chromatin. 
Biitschli doubts if the “reserve” granules are formed of a carbohy- 
drate, but he believes that the action of iodine solutions on material 
which has been kept for a long time in alcohol points to the presence of 
glycogen in the Cyanophycee. . 
The central body Biitschli holds to be a nucleus. The objections to 
such a view he deals with in detail and points out that the absence of 
indirect division occurs also in the macro-nuclei of Infusoria, in nuclei of 
Amebe, Dinoflagellata and Euglena, while he urges in answer to 
Zacharias, who could find no nuclein in the central body either during 
rest or division of the latter, that this does not diminish the suspicion 
concerning the poverty of our micro-chemical methods. 
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