a, gs Te 
= Y 
mn : = / 
1898-99. | ON THE CYTOLOGY OF NON-NUCLEATED ORGANISMS. 455 
describe the method used. The material, after thorough fixation with 
alcohol, was washed free from the latter with distilled water, then put in 
the nitric-molybdate solution for three to six hours at a temperature of 
35° C., and finally, after quickly washing in water, treated for a few 
minutes with a one per cent. solution of phenylhydrazin hydrochloride, 
which converts the phospho-molybdate into a bluish-green compound, 
this colour reaction thus indicating the original distribution of the 
organic phosphorus. To provide against the phosphorus demonstrated ~ 
being that of lecithin, the material was extracted with hot alcohol in a 
Soxhlet apparatus for five hours. 
The other methods resorted to included : digestion of fresh material 
with artificial gastric juice and with alkaline solutions of trypsin, staining 
of fresh material for twenty-four hours with acetic-methyl green and 
subsequent fixation by saturated solutions of picric acid and the treat- 
ment of fresh material with solutions of Ehrlich’s hematoxylin. The 
digested material was similarly stained and all the preparations were 
mounted in glycerine. 
III—THE LIVING CELL IN THE CYANOPHYCEA. 
In the larger species of Cyanophycee the cell substance readily 
reveals the existence of two zones, one, peripheral, coloured blue-green, 
the other, central, containing granules and, but for the shimmer of blue- 
green of the outer zone through which it is seen, colourless. In Fig. 
I the division of the cytoplasm is very clearly indicated. The smaller 
the cell the greater is the difficulty with which the central uncoloured 
zone is observed and in those species in which the cell is long and 
narrow, ¢.g., Wicrocoleus terrestris or Oscillaria tenerrima, the central zone 
cannot be seen, it being of such minute dimensions that its presence is 
masked by the outer zone. 
Whenever it can be distinctly seen the central zone, or, as it is usually 
called, the central body, is found to contain granules which vary in size 
and numbers, but these most frequently appear in the outer portion of 
the central body and in some cases the inner portion of the latter may 
be completely free from them. Apart from the granules the central 
body is uniform in structure, appearing to possess a vesiculated structure 
which comes out quite distinctly in Osczllaria princeps. The vesicles in 
the latter are not closely aggregated and are separated from one another 
by coarse trabecule of a colourless, hyaline, plasma-like substance in 
which excessively minute, punctiform appearances suggest a granulation 
