1898-99. | ON THE CYTOLOGY OF NON-NUCLEATED ORGANISMS. 457 
central body, appear blue-green, but that they are uncoloured can be 
determined readily by causing the spores to burst, when the granules, 
becoming free, appear ‘unpigmented. These, to a certain extent, 
correspond, as will be shewn below, to the “cyanophycin ” granules of 
Palla and Borzi. “Cyanophycin” granules are usually abundant in the 
Oscillarie@, being found in a row at each end of the cell adjacent to the 
transverse septum, but in JZzcrocoleus terrestris, in Tolypothrix, Scytonema 
and Lyngbia, they are distributed throughout the substance of the 
peripheral zone. 
IV.—FIXED PREPARATIONS OF CYANOPHYCE&. 
When trichomes of Osczllarta Froehlichiz have been hardened in picric 
acid for several days and stained with picrocarmine and hematoxylin, 
the preparations contain isolated elements like those represented in 
Figs. 2 and 3. Similar preparations are obtainable from this form 
when corrosive sublimate is used as.a fixative agent, but the vesiculation 
of the cytoplasm, while distinct, is less clearly demonstrated than in 
picric acid preparations. Like preparations may be obtained with 
Flemming’s fluid. 
The cytoplasm has the structure which Riitschli claims obtains in it. 
The character of the vesiculation is, however, not what he describes nor 
does every trichome even in a picric acid preparation present a similar 
vesiculation. In all, on the other hand, the vesiculation of the central 
body appears of a finer character than that in the peripheral zone. 
When the spaces in the cytoplasm are, as is the case in some trichomes, 
very minute it would be difficult to determine whether vesiculation 
obtains, were it not that some threads show all the stages between this 
condition and that in which the vesiculation is distinctly pronounced. 
In the latter the walls separating the vesicular cavities have a mem- 
branous appearance with an indefinite character to their borders. 
Owing to the more minute character of the vesicles in the central 
body, the latter has a compact appearance, and as it stains with 
hematoxylin and other dyes some what deeply and uniformly it is thereby 
brought out sharply in contrast with the cytoplasm of the peripheral 
zone. There is usually not a distinct line of demarcation between them, 
but the vesiculation of the central body may pass almost abruptly into that 
of the peripheral zone. The vesicles in the latter are elongated, tending 
to extend from the central body to the membrane of the cell. In con- 
sequence of the peripheral layer being thicker at the sides than it is 
