460 TRANSACTIONS OF THE CANADIAN INSTITUTE. [Vov. VI. 
0.5 per cent., equal volumes) for five minutes to give the Prussian-blue 
reaction, or with aqueous hematoxylin (0.5 per cent. solution) for a few 
minutes, preparations are obtained which show quite distinctly that the 
central body contains a substance in which “masked ” iron is held. At 
the same time one species of the granules also may show the presence 
of “ masked” iron and this may be observed when the glycerine sulphide 
method is used. The Prussian-blue reaction exhibited by the central 
body is a uniform one and the depth of the blue colour depends on the 
thickness of the cell but it is unmistakable in every case. The bluish- 
black given by the haematoxylin is similarly marked. It is to be noticed, 
however, in all these cases that the blue of the Prussian-blue reaction 
and the bluish-black of the hematoxylin are not limited to the central 
body, for a lighter blue or a faint haematoxylin reaction may be found 
in that part of the peripheral zone next to the central body, and very 
often in such preparations it is not possible to say where the line of 
separation is. This is the case sometimes in Oscellarza Froehlichit and 
if the cell discs are seen from their flat surface the blue colour is marked in 
the centre but gradually diminishes towards the periphery and is obscure 
or absent in the outer part of the peripheral zone. A similar distribution 
of the “masked” iron in the peripheral zone is shown by the glycerine- 
sulphide method, but the reaction is not as decisive for minute quantities 
of iron. 
There can, therefore, be no doubt that the central body specially, and 
a portion of the peripheral zone immediately about it in a very much 
less degree, contain “ masked ” iron. 
The reaction for organic phosphorus is not less distinct. Treatment of 
trichomes, hardened in alcohol, with a nitric acid solution of ammonium 
molybdate for several hours at a temperature not exceeding 35° C., then 
with dilute nitric acid for a few minutes and afterwards with a two per 
cent. freshly prepared solution of phenylhydrazin hydrochloride for from 
three to five minutes, gave definite indications of the presence of phos- 
phorus in the production of a dull greenish-blue reaction in the central 
body specially and only faintly in the cytoplasm of the peripheral zone. 
Certain granules also give the reaction deeply as in the case of the iron 
reaction. The reaction in the central body is, as stated, a marked one, 
and it is uniform, shading at the margins of the central body into the 
feeble reaction of the peripheral zone. That the reaction is due to 
organic phosphorus and not to inorganic compounds of this element is 
indicated by the fact that the nitric-molybdate reagent brings out the 
full reaction after two hours at the earliest, and then only gradually. 
The presence of “ masked” iron and organic phosphorus in the central 
