1898-99. | ON THE CYTOLOGY OF NON-NUCLEATED ORGANISMS. 465 
V.—THE GRANULES IN THE CYANOPHYCE. 
The granules, according to Biitschli, Palla and Nadson, are of two 
kinds, one which stains red-violet with Delafield’s hematoxylin and 
situated, according to Palla, on the periphery of the central body, 
according to Biitschli on the central body or in its more peripheral 
portions, according to Nadson, in the central body only; the other which 
stains blue or blue-violet, to be found chiefly adjacent to the transverse 
septa. The latter class of granules Biitschli claims is not affected by 
hamatoxylin staining, and he usually demonstrated their presence with 
eosin. Zacharias also has described the occurrence of two kinds of 
granules, and the writer showed that one kind, those which are described 
by Biitschli as staining red-violet with hematoxylin, contain a “masked” 
iron compound, while the other kind of granules are, as a rule, free from 
“ masked” iron. Hieronymus, Deinega and Fischer, however, claim that 
there is only one kind of granules present. The first-named observer 
holds that two kinds of granules appear to be present because of the 
methods of preparation, but that if fixed cells are treated with ammonia 
vapour all the granules colour dark blue with hematoxylin and further 
that all dissolve in dilute acids although not equally readily. Fischer, 
on the other hand, maintains that the granules do not disappear in 
artificial digestion (with pepsin and hydrochloric acid) and further that, 
if they are treated with dilute soda solutions, all stain blue with hama- 
toxylin. He also finds that in some forms all the granules are stained 
either blue or red, while in other preparations some of the granules stain 
blue, the remainder red. He claims that the method of fixation employed 
greatly varies the capacity of some of the granules, notably those which 
stain blue with hematoxylin, to absorb the staining compound, and he 
concludes that staining is due to differences, not of chemical properties, 
but of physical condition produced by the fixing reagent. The granules 
which Deinega found were soluble in acids and stained specially with 
picrocarmine. 
I have made a lengthy examination of the granules which occur in the 
Cyanophycee and | find that. while in some species one kind only may 
be present, yet in other forms two distinct types of granules occur. 
Illustrations of these two types may be found in the Oscz/larie, in 
Tolypothrix, Scytonema and Microcoleus terrestris. The best method of 
demonstrating them is to harden the material in picric acid or corrosive 
sublimate, or even in alcohol alone, and then stain first with picrocar- 
mine and afterwards with dilute Ehrlich’s hematoxylin. The latter 
