1898-99. | ON THE CYTOLOGY OF NON-NUCLEATED ORGANISMS. 479 
vesicles are separated from each other by a thin film of cytoplasm, and 
here and there, and especially at the nodal points in these films, one 
observes, in heematoxylin preparations, granules which are more deeply 
stained than the rest of the cytoplasm. Sometimes these granules are 
more or less drawn out in the films between the vesicles, and then the 
central cytoplasm may take on the character of the central body of 
Biitschli. Of the actual existence of such a central body there is not 
the slightest evidence. 
In the “comma” forms the granules are very much fewer, and fre- 
quently smaller. In the “cocci” they are rarely visible, and minute 
lightly stainable granules make their’ appearance at the periphery 
of the vesicles (Fig. 59, a, 6, and c.). 
In the “spirillum,” as well as in the “comma” form, the compounds of 
“masked” iron and organic phosphorus are, apart from the granules, 
faintly diffused throughout the cytoplasm,sometimes apparently less abun- 
dant in the central portions of the cells, and definitely indicated in the 
peripheral layer. The granules give a slightly more marked reaction for 
“masked” iron as well as for organic phosphorus, and this fact, com- 
bined with their capacity for taking up hematoxylin, would seem to 
indicate that they are formed of a compound analogous to chromatin. 
The difference between the structure of the threads, and that of the 
“spirilla” and the “comma” forms in regard to granules, may be due to 
some inherent difference in the process of nutrition in the two different 
types, but it may also be explained on the view of Winogradsky that 
these types are not genetically connected, that they perons to different 
species of Sulphur Bacteria. 
Whether this is correct or not does not affect the question that, in all 
these types of structure, there is nothing to simulate a nucleus, even of 
the most rudimentary description. It can scarcely be contended that 
the granules, the affinity of whose substance to chromatin is shown by 
their containing, in a small degree, “masked” iron and organic phos- 
phorus, are morphologically of the value of nuclei, or even of nucleoli, 
as Mitrophanow appears to claim. 
