486 TRANSACTIONS OF THE CANADIAN INSTITUTE. [VoL. -VI. 
tures: one of which he calls the nuclear body, while he terms the 
other the nuclear vacuole. The nuclear body, which is the nucleus of 
Moeller, and of his own earlier observations, is homogeneous, 
but surrounded more or less completely by granules, and which, with 
low powers of magnification, give it a granular appearance. It is, in 
actively growing cells, usually in close contact with the cell wall, but it 
may, in a few cells, be more centrally placed. In relation frequently 
to this nuclear body there are granules, first described by Hieronymus, 
some of them of an oily nature, others of a proteid character, sometimes 
erouped about the nuclear body, sometimes in its immediate neighbor-. 
hood or distributed throughout the cell. At times these form a coiled 
thread. The nuclear vacuole, which is in contact with the nuclear body 
in growing cells, contains chromatin, sometimes in the form of granules, 
sometimes in the form of anetwork, sometimes as an irregularly shaped 
mass attached to the wall of the vacuole by fine threads. In some cells 
all the chromatin substance appears to reside in the vacuole ; in others 
it is diffused through the protoplasm, and in some cells again it appears 
in the nuclear body. The vacuole Wager regards as the nucleus of 
Janssens and Leblanc, and the nucleolus of these observers con- 
stitutes his nuclear body. The nuclear vacuole may persist but for 
a short time. After fermentation has proceeded for some hours it dis- 
appears, and its place is occupied by a granular network in contact with 
the nuclear body. The vacuoles seem to arise by fusion of minute 
vacuoles which develop in connection with what appears to be chroma- 
tin granules. 
In regard to division, Wager found that the nucleolus (the nuclear 
body) is separated from the bud by the vacuole, which, as the bud deve- 
lops, begins to pass into it. At the same time, the nucleolus makes its 
way to the base of the opening, and there, or in the neck, at once begins 
to elongate and constrict for division. The vacuole at this time divides, 
but not completely or equally, the smaller portion being found in the 
daughter cell, both parts remaining connected by a granular thread. 
The divisions of the nuclear body are nearly or quite equal, and one of 
them makes its way into the daughter cell. When the nucleolus 1s in 
the neck, the constriction takes place with the ends in the mother and 
daughter cells. When there is no vacuole, the granular network in 
contact with the nuclear body undergoes division into two more or less 
equal portions, either in the mother cell or in the neck of the bud. The 
eranules which the young bud thus receives seem to develop, in some 
way, the vacuoles which form the single large nuclear vacuole. 
In sporulation the nuclear vacuole disappears, or its place is taken by 
