add 
1898-99. | ON THE CYTOLOGY OF NON-NUCLEATED ORGANISMS, 495 
stained with very dilute solutions of haematoxylin, the corpuscles may 
be unstained, or stained no more deeply than the cytoplasm generally, 
(Fig. 28). 
In ordinary cultures of S. Ludwigd the garland-like structure may 
be so infrequently present in its typical form as to be overlooked. Then 
in many cells granules like those found by Wager and Bouin in 
S. cerevisi@ may be observed, This illustrates how much, as regards 
structure, is dependent on the mode of cultivation of the yeast cell. 
When yeast cells, hardened in alcohol, were submitted at 37°C. to 
digestion in artificial gastric juice, made by dissolving some glycerine 
extract of pepsin in a 0.2 per cent. solution of hydrochloric acid, 
after forty-eight, seventy-two, and ninety-six hours there were very 
few evidences of the occurrence of corpuscles remaining, nor was there 
any stainable substance left in the cytoplasm. The corpuscles seem 
to be affected very much by the digestive solution, for even when 
the cells were deeply stained with the iron-alum hematoxylin, cor- 
puscles were only rarely found, and then they appeared with a large 
vacuole in their interior (Figs. 39 and 41), or to have lost their 
stainable substance (Fig. 40). These traces of the corpuscle also 
disappeared after treatment of the preparations for 24 hours with 
a O.I per cent. solution of potassic hydrate. The results of the treat- 
ment with artificial gastric juice seem to indicate that the stain- 
able substance in the cytoplasm and in the corpuscles is different from 
the chromatin of the nuclei of other organisms, which is unaffected by 
this fluid. 
Similar results were obtained when fresh cultures of S. cerevisz@, in 
Pasteur solution, were digested for forty-eight hours or more in artifi- 
cial gastric juice, after which the cells were fixed with iodine solutions 
and hardened in alcohol. Only very rarely in these, even after the most 
careful application of the various methods of staining, and especially 
of the iron-alum hematoxylin process, did I succeed in finding traces of 
a corpuscle. The cells of such preparations seem to have lost their 
power to stain with Ehrlich’s and Delafield’s hematoxylin solutions, but 
to have increased their affinity for eosin. 
The micro-chemical reactions are quite decisive as regards the rela- 
tionship of the stainable substance. When the glycerine-ammonium 
sulphide method is employed to demonstrate the organic iron in cells of 
S. Ludwigit and S. cerevisig, the results obtained after ten days at the 
latest distinctly demonstrated the presence of “ masked” iron in the 
one or more corpuscles which may be present in each cell in the walls 
