1898-99. | ON THE CYTOLOGY OF NON-NUCLEATED ORGANISMS. 497 
mation of the chromosomes (Fig. 23). In other preparations, one may 
obtain cells in which a series of vacuoles is found in close contact with 
each other, and all surrounded by a substance which stains deeply in 
hematoxylin (Figs. 22 and 24). Rarely one sees structures like those 
illustrated in Figs. 26 and 27. In Fig. 25, there are in mother and 
daughter cells structures allied in general form to those found in Figs. 
22 and 24. 
A somewhat similar instance of vacuolation of chromatin-like masses 
may be observed in mycelium-like threads developing sometimes 
with the cells of S. Luadwzg7i in sap cultures. In these the vacuoles 
vary from an almost infinitesimal size to that of extraordinary dimen- 
sions (Fig. 58). The largest ones appear to be formed of a large num- 
ber of vacuoles fused through the rupture of the more centrally placed 
partitions (Fig. 58¢). Ina few cases the fused vesicles may form a very 
large structure, presenting some resemblance to a nucleus (Fig. 57). 
Whether these structures are formed of that variety of chromatin 
which is to be found in yeast cells cannot be decided as yet. The cells 
containing them are so few in any preparation made with the glycerine- 
ammonium sulphide method to show the distribution of organic iron, or 
with the nitric-molybdate reagent to determine the occurrence of 
organic phosphorus, that they must only very rarely be observed. In 
only one sulphide preparation did I see a cell which appeared to contain 
a structure like one of those in question (Fig. 52). In this case the iron 
reaction of the substance forming the structure was quite marked. It 
would seem to indicate that these structures are formed of yeast 
chromatin. 
There can be no question about the nature of these structures. They 
certainly are not nuclei, either normal or degenerated. They owe their 
form and arrangement to a property of chromatin which, I believe, has 
not hitherto been regarded as characteristic of it. In the Cyanophycee, 
as already described, the chromatin-like substance not dissolved in the 
“central body” forms spherules, in the centre of each of which may be 
found a vacuole (Figs. 4, 6, and 11). In the Foraminifer, Cadcztuba poly- 
morpha Roboz,' the nuclear chromatin before division, at first homo- 
geneous, undergoes extensive vacuolation, and upon this process 
division depends. Indeed the structure of the nucleus ordinarily would 
appear to depend on the inherent power of chromatin to produce vesi- 
culation or vacuolation, with the formation ultimately of a reticular 
. 
t F. Schaudinn, ‘‘ Untersuchungen an Foraminiferen. I. Calcituba polymorpha Roboz,”’ Zeit. fiir Wiss 
Zool., Vol. LIX, p. 191, 1895. 
