500 TRANSACTIONS OF THE CANADIAN INSTITUTE. [VoL. VI. 
for organic iron with the glycerine-sulphide method. Perhaps this dis- 
tribution may be explained by supposing that the first cytoplasm to pass 
out into the developing bud is richer in chromatin-like substance. 
Quite different is the action of the corpuscle in sporulation. Here, 
however, the cytoplasm also acts differently. When the cell is ready to 
sporulate the chromatin dissolved in the cytoplasm begins to concen- 
trate in a zone about the corpuscle, the diameter of the zone diminish- 
ing as this stage advances, while the corpuscle appears to lose its 
distinctness. The concentration advances until all of the cytoplasmic 
chromatin is collected in a very narrow zone about the corpuscle which, 
in some cases, may appear very finely granular. At this stage occurs 
an elongation of the corpuscle and its enclosing body of cytoplasmic 
chromatin, the elongation rarely being to the full length of the cell. 
The central portion constricts or becomes more and more slender, until 
separation of the more or less rounded extremities occurs. Thus two 
corpuscles are formed, each with a very narrow enclosing zone of 
cytoplasmic chromatin. Each of these now elongates and divides as 
the parent structure does. In the second division, however, the cyto- 
plasmic chromatin seems to disappear, or perhaps is taken up into the 
corpuscle. 
My observations on the whole agree with those of Wager, but I 
have never been able to find the granules which form in the corpuscle 
or nucleolus immediately before and during its elongation, as described 
and illustrated by that observer. Nor can I corroborate his view that 
the nuclear vacuole, which exists in the cell previous to sporulation, 
divides and redivides many times, thus distributing the chromatin 
through the cytoplasm, which has, in consequence, a delicate foam-like 
structure. So far as my observations go, the chromatin in the cyto- 
plasm before the stage of sporulation commences, is not different in its 
character or distribution from that found in the ordinary yeast cell, for 
example, during budding. 
According to Janssens and Leblanc, the act of sporulation is preceded 
by a division of the nucleus and its nucleolus, followed by a fusion of 
the two nuclei thus produced. These authors believe that this fusion 
or conjugation constitutes sexual fertilization. They find that the two 
nuclei formed disappear, and in their place, one only, whose nucleolus 
is large and distinct, is observed. It is rather difficult to accept this 
interpretation. What they claim to have observed as constituting a 
nucleus may, as I have pointed out, be found in some cells only, and their 
nucleolus is the corpuscle, two or more examples of which may some- 
ater i 
