1898-99. | ON THE CYTOLOGY OF NON-NUCLEATED ORGANISMS. 503 
SUMMARY. 
1. In Saccharomyces the cytoplasm is usually finely reticulated, and 
contains one or more vacuoles. It takes a diffuse stain with hzma- 
toxylin, and gives a diffuse reaction for “masked” iron and organic 
phosphorus. 
2. In addition to the chromatin-like substance diffused throughout 
the cytoplasm, there is usually a more or less homogeneous, spherical 
body in the cell, the corpuscle, the “nucleus,” “ nucleolus,” and “nuclein 
body” of various observers, which stains specially with haematoxylin, 
and gives the reactions for “masked” iron and organic phosphorus, but 
does not stain with acetic-methyl green. This body is neither a nucleus 
nor a nucleolus. Several examples of it, though of small size, may be 
present in a cell. On the other hand, cells are found without a trace of 
a corpuscle. 
3. The chromatin-like substance differs from the chromatin of higher 
animal and vegetable cells in being soluble in artificial gastric juice. 
4. When budding begins, the corpuscle, if placed adjacent to the 
point where the bud is developing, becomes elongated and constricted 
in its middle portion. One end of the elongated structure may be 
forced into the neck of the bud, and when the constriction is completed 
by separation of the two halves, the daughter cell may thus receive a 
corpuscle. Both daughter corpuscles may pass into the bud, leaving the 
mother cell without a corpuscle. A complete bud may be formed with- 
out such a division of the corpuscle taking place, and thus the daugh- 
ter cell may commence independent life without a corpuscle. . 
5. In sporulation the cytoplasmic chromatin collects in the immediate 
neighborhood of the corpuscle, which also undergoes certain granular 
changes, then elongates with constriction in its central part. Each of 
the two daughter corpuscles thus formed repeats this process of division 
one or more times, the daughter corpuscles resulting ultimately forming 
the corpuscles of the spores. 
6. The division of the corpuscles in budding is a purely mechanical 
result, and is not essential to the formation of the bud. The division 
preparatory to sporulation is apparently a functional act. It is not of 
the nature of true karyokinesis, and it may be compared to the division 
of the nucleolus in Euglena viridis, 
