1904-5.] ON THE ABSORPTION OF FaT IN THE INTESTINE. 243 
cells, but in kittens the fat may pass not only through the cytoplasm, but 
also through the intercellular spaces to parenchyma. 
The writer had, before Kischensky’s paper came to his knowledge, 
begun the study of the absorption of fat and had, through Michaelis’ 
observations, been led to employ scarlet red as a micro-chemical reagent 
for fat. With this and other means he carried out the work, and when 
it was completed, arrived at results which are, in many respects corrobo- 
rative of those obtained by Kischensky, but were obtained from a larger 
number of vertebrate forms. These results are now published in the hope 
that they will serve in some degree to advance the solution of the question 
of the mode of absorption of fats. 
II.—METHODS OF STUDY. 
During the first five months guinea pigs were almost exclusively used. 
Sometimes they were kept from all food for as many as four days before 
they were employed, while others were not so treated. They were fed 
on egg yolk diluted sufficiently with tap water to allow the mixture being 
drawn up into a pipette used for feeding, and fifteen to twenty cubic 
centimetres of this were given to each three times a day. At the outset 
of the investigation many of the animals were fed on diluted yolk to 
which one cubic centimetre of dialysed iron was added on each occasion. 
Others again were given only pure olive oil. Some were fed only once, and 
killed from three to six hours afterwards, others were fed one, two or three 
days, while a few were continued on this diet alone until death ensued. 
The rest of the animals employed were invariably killed with chloroform, 
the abdomen was at once opened, the duodenum exposed, and pieces 
about a centimetre and a half long, and one half a centimetre broad taken 
from different portions, were placed in Flemmings Fluid for about twelve 
hours. The composition of this fluid was :— 
oO. I per cent. chromic acid, 15 parts. 
2. per cent. osmic acid, 4 parts. 
One part glacial acetic acid was added to nineteen parts of the above 
when about to be used. The specimens were then washed in running 
water for half an hour, placed in fifty per cent. alcohol, after about twelve 
hours in seventy per cent. for twenty-four hours, then in ninety-five per 
cent. for another twenty-four hours, and finally into absolute alcohol for 
periods varying from twelve to seventy-two hours. When it was con- 
sidered necessary the absolute alcohol was changed and fresh added. 
