244 TRANSACTIONS OF THE CANADIAN INSTITUTE. (VoL. VIII. 
Some of the specimens were then passed through chloroform into paraffin 
while others were infiltrated with celloidin before being imbedded in 
paraffin. Owing, however, to the presence of chromic acid in the fixing 
agent the proteids are so coagulated that not enough is left unchanged in 
the sections to cause the latter to adhere, so that in most cases a small 
amount of collodion and oil of cloves had to be employed for attaching 
the section to the slide. In a few instances the sections were made adher- 
ent with albumen and glycerine. 
The preparations were treated after the iron-alum hematoxylin 
method, followed by eosin staining. Numerous other stains were used 
but this method gave by far the best results so that it was the one chiefly 
used. With this stain the nuclei stand out as blue black, the cytoplasm 
takes the eosin, while the fat is black or, if not very abundant and seen 
with the diaphragm of the microscope well open, a brownish tinge is 
evident. 
With the view of investigating more closely the striated border I 
experimented with other fat stains, and finally obtained what I required 
in scarlet red (Scharlach Rot). This stain is insoluble in water, but 
soluble in seventy to eighty per cent. alcohol. It is also soluble in soap 
solution, but insoluble in glycerine or chloral hydrate, five per cent In 
using it one must be careful to get a solution of proper concentration, else 
the results are very unsatisfactory. It requires to be shaken from time 
to time with seventy, or even eighty per cent. alcohol for three or four days 
and then filtered. Before using it should be tested on a little olive oil 
smeared on a slide, when, if good, it will give a deep red reaction to the 
globules in about one minute. 
In my first experiments I used guinea pigs fed for about three days 
upon olive oil. They were then killed with chloroform about three hours 
after the last feeding, the abdomen opened, a piece of the duodenum 
was put into ten per cent. formalin for about forty-eight hours, 
then frozen with carbon dioxide spray, and sections made about six-seven 
microns thick. These were put into the scarlet red solution for about 
forty-five seconds, then transferred to tap water for a few seconds and 
mounted in glycerine, the edges of the cover slip being later smeared with 
melted paraffin. 
