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1908-9.] MICROCHEMISTRY OF STRIATED MUSCLE. i 419 
EXPLANATION OF FIGURES ILLUSTRATING THE 
MICROCHEMISTRY OF STRIATED MUSCLE. 
Fig. 1.—Thorax muscle,—Bee, fixed in 4 per cent. formalin and stained in 
hematoxylin and eoxin. The more deeply stained sheaths enclosing portions of the 
fibrils and fragments of the adherent myoplasm x 1000. 
Figs. 2—6.—Thorax muscle,—Bee fixed in 4 per cent. formalin and stained 
in Scarlet Red, 10 to 15 minutes. In Fig. 2, the part of the fibre stained orange 
represents the myoplasm x 1000. 
Fig. 7.—Wing muscle, Tenebrio obscurus. Fixed in 2 per cent. formalin stained 
with Scarlet Red, 10 minutes x rooo. 
Fig. 8.—Wing muscle, 7Zenedbrio obscurus. Fixed in 2 per cent. formalin and 
treated with Scarlet Red, 75 hours, shewing the fat confined to the dim band 
x 1000. 
Fig. 9—10—Wing muscle, Tenebrio molitor. Fixed in 2 per cent. formalin and 
stained with Scarlet Red, 75 hours. In both the fat occurs chiefly in the light bands 
xX 1000. 
Fig. 11.—Wing muscle, Zenebrio molitor. Fixed in 2 per cent. formalin, stained 
with Scarlet Red, 125 hours. A deep reaction obtains in the light band, but the 
central zone, which marks the position of Dobie’s Line is free from fat. In the dim 
band a faint reaction occurs along Hensen’s Line x 1000. 
Fig. 12.—Wing muscle, TZexebrio obscurus. Fixed in 2 per cent. formalin and 
stained in Scarlet Red, 125 hours, shewing a fibre partly relaxed and partly con- 
tracted x 1000. 
Fig. 13.—Thorax muscle, Bee. Fixed in 4 per cent. formalin, stained with 2 
per cent. aqueous hzmatoxylin, 36 hours. x r1ooo. 
Fig. 14.—Thorax muscle, Bee. Fixed in 4 per cent. formalin and treated with 
absolute alcohol, and then sulphuric acid alcohol, 24 hours, stained with 2 per cent. 
aqueous hzmatoxylin x 1000. 
Fig. 15.—Preparations of same shewing the reaction for organic iron which obtains 
in the nuclei x 1000. 
N 
Fig. 16.—Muscle, Lachnosterna fusca. — AgNO,+1.5 per cent. HNO,, 48 hours, 
x 1000. We) 
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Fig. 17.—Muscle, Wasp. — AgNO,+1.5 per cent. HNO,, 5 days. The faintly 
10 
marked reaction represents Hensen’s Line x 1335. 
N ‘ 
Fig. 18.--Muscle, Lachnosterna fusca. — AgNO,+1.5 per cent. HNO,, 48 hours, 
10 
showing the distribution of the chlorides extending in horizontal plane across the fibre 
x 1000. 
